Literature DB >> 8234492

Transformation of Mycoplasma pneumoniae with Tn4001 by electroporation.

C T Hedreyda1, K K Lee, D C Krause.   

Abstract

Mycoplasma pneumoniae was transformed with the Staphylococcus aureus transposon Tn4001 by electroporation. A transformation frequency of 10(-3) to 10(-5)/colony-forming unit was observed using 30.0 microgram plasmid DNA and 10(7)-10(8) M. pneumoniae colony-forming units. DNA hybridization analyses using standard and pulsed field agarose gel electrophoresis confirmed chromosomal insertion of the transposon, apparently by a transpositional mechanism into random sites. These studies demonstrate the functionality of Tn4001 in M. pneumoniae and suggest its potential as a genetic tool in this mycoplasma.

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Year:  1993        PMID： 8234492     DOI： 10.1006/plas.1993.1047

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  39 in total

1.  Loss of co-chaperone TopJ impacts adhesin P1 presentation and terminal organelle maturation in Mycoplasma pneumoniae.

Authors:  Jason M Cloward; Duncan C Krause
Journal:  Mol Microbiol       Date:  2011-06-23       Impact factor: 3.501

2.  Use of fluorescent-protein tagging to determine the subcellular localization of mycoplasma pneumoniae proteins encoded by the cytadherence regulatory locus.

Authors:  Tsuyoshi Kenri; Shintaro Seto; Atsuko Horino; Yuko Sasaki; Tsuguo Sasaki; Makoto Miyata
Journal:  J Bacteriol       Date:  2004-10       Impact factor: 3.490

3.  Targeted chromosomal knockouts in Mycoplasma pneumoniae.

Authors:  Radha Krishnakumar; Nacyra Assad-Garcia; Gwynedd A Benders; Quang Phan; Michael G Montague; John I Glass
Journal:  Appl Environ Microbiol       Date:  2010-06-11       Impact factor: 4.792

4.  GAA trinucleotide repeat region regulates M9/pMGA gene expression in Mycoplasma gallisepticum.

Authors:  L Liu; K Dybvig; V S Panangala; V L van Santen; C T French
Journal:  Infect Immun       Date:  2000-02       Impact factor: 3.441

5.  HMW1 is required for stability and localization of HMW2 to the attachment organelle of Mycoplasma pneumoniae.

Authors:  Melisa J Willby; Mitchell F Balish; Stephanie M Ross; Kyungok K Lee; Jarrat L Jordan; Duncan C Krause
Journal:  J Bacteriol       Date:  2004-12       Impact factor: 3.490

6.  Mutant analysis reveals a specific requirement for protein P30 in Mycoplasma pneumoniae gliding motility.

Authors:  Benjamin M Hasselbring; Jarrat L Jordan; Duncan C Krause
Journal:  J Bacteriol       Date:  2005-09       Impact factor: 3.490

7.  Synthesis, stability, and function of cytadhesin P1 and accessory protein B/C complex of Mycoplasma pneumoniae.

Authors:  Robert H Waldo; Duncan C Krause
Journal:  J Bacteriol       Date:  2006-01       Impact factor: 3.490

8.  Identification of a virulence-associated determinant, dihydrolipoamide dehydrogenase (lpd), in Mycoplasma gallisepticum through in vivo screening of transposon mutants.

Authors:  P Hudson; T S Gorton; L Papazisi; K Cecchini; S Frasca; S J Geary
Journal:  Infect Immun       Date:  2006-02       Impact factor: 3.441

Review 9.  Gene transfer to plants by electroporation: methods and applications.

Authors:  Ibrahim Ilker Ozyigit
Journal:  Mol Biol Rep       Date:  2020-04-02       Impact factor: 2.316

10.  Unmarked insertional mutagenesis in the bovine pathogen Mycoplasma mycoides subsp. mycoides SC: characterization of a lppQ mutant.

Authors:  Carole Janis; Daniela Bischof; Géraldine Gourgues; Joachim Frey; Alain Blanchard; Pascal Sirand-Pugnet
Journal:  Microbiology       Date:  2008-08       Impact factor: 2.777
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