| Literature DB >> 823295 |
L Enjuanes, A L Carrascosa, E Viñuela.
Abstract
African swine fever (ASF) virus was grown either in swine macrophages or in VERO cells and purified free of cell DNA. Virus DNA was isolated from virions as a molecule with a sedimentation coefficient of 60S and a contour of 58 +/- 3 mum. .these two values give a mol. wt. of 102 +/- 5 X 10(6) and 107 +/- 5 X 10(6), respectively, for the genome of ASF virus. Denatured DNA fragments from ASF virus reassociate with a C0t1/2 value of 0-60 +/- 0-05 MS, which compared with the corresponding value for T4 DNA gives for the molecular mass of ASF virus DNA a value of 102 +/- 8 X 10(6) daltons. Only virus DNA is synthesized ASF virus-infected swine macrophages.Entities:
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Year: 1976 PMID: 823295 DOI: 10.1099/0022-1317-32-3-479
Source DB: PubMed Journal: J Gen Virol ISSN: 0022-1317 Impact factor: 3.891