Literature DB >> 822884

Counting the genes for stabel RNA in the nucleus and chloroplasts of Euglena.

D J Gruol, R Haselkorn.   

Abstract

Improved procedures for counting the genes for ribosomal and 4 S RNA in the nucleus and chloroplasts of Euglena are described. These procedures exploit (a) the chloroplast-free strain ZHB, (b) the flotation method for purifying chloroplasts, (c) Hg2+/Cs2SO4 density gradients to fractionate large quantities of DNA and (d) more efficient conditions of DNA - RNA hybridization particularly for 4 S RNA. We find that the average Euglena cell in an exponentially growing culture contains 800 nuclear genes for each cytoplasmic rRNA and 800-880 chloroplast genes for chloroplast rRNA. Each chloroplast chromosome of molecular weight 92 - 10(6) contains two genes for each rRNA. The number of nuclear genes for 4 S RNA is approx. 760. The total number of chloroplast genes for 4S RNA is approx. 10 000, or 22-25 on each chloroplast chromosome.

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Year:  1976        PMID： 822884     DOI： 10.1016/0005-2787(76)90098-8

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  7 in total

1.  Analysis of intergenic spacer transcripts suggests 'read-around' transcription of the extrachromosomal circular rDNA in Euglena gracilis.

Authors:  S J Greenwood; M N Schnare; J R Cook; M W Gray
Journal:  Nucleic Acids Res       Date:  2001-05-15       Impact factor: 16.971

2.  Transfer RNA genes ofZea mays chloroplast DNA.

Authors:  R F Selden; A Steinmetz; L McIntosh; L Bogorad; G Burkard; M Mubumbila; M Kuntz; E J Crouse; J H Weil
Journal:  Plant Mol Biol       Date:  1983-05       Impact factor: 4.076

3.  The nucleotide sequences of the genes coding for tRNAArgUCU, tRNAArgACG and tRNAAsnGUU on Spirodela oligorhiza chloroplast DNA.

Authors:  R J Keus; N J Stam; T Zwiers; H T de Heij; G S Groot
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

Review 4.  Structure and evolution of organelle genomes.

Authors:  D C Wallace
Journal:  Microbiol Rev       Date:  1982-06

5.  Nuclear rDNA in Euglena gracilis: paucity of chromosomal units and replication of extrachromosomal units.

Authors:  P Ravel-Chapuis
Journal:  Nucleic Acids Res       Date:  1988-06-10       Impact factor: 16.971

6.  Extrachromosomal circular nuclear rDNA in Euglena gracilis.

Authors:  P Ravel-Chapuis; P Nicolas; V Nigon; O Neyret; G Freyssinet
Journal:  Nucleic Acids Res       Date:  1985-10-25       Impact factor: 16.971

7.  Fractionation and identification of Euglena gracilis cytoplasmic and chloroplastic tRNAs and mapping of tRNA genes on chloroplast DNA.

Authors:  M Kuntz; M Keller; E J Crouse; G Burkard; J H Weil
Journal:  Curr Genet       Date:  1982-10       Impact factor: 3.886
  7 in total

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