Literature DB >> 8226629

Oxygen-regulated in vitro transcription of Rhizobium meliloti nifA and fixK genes.

J M Reyrat1, M David, C Blonski, P Boistard, J Batut.   

Abstract

Oxygen concentration regulates the expression of nitrogen fixation genes in the symbiotic bacterium Rhizobium meliloti. We demonstrate that two proteins, FixL and FixJ, that belong to the two-component family of regulatory proteins are necessary and sufficient for oxygen-regulated in vitro transcription of the two key regulatory genes, nifA and fixK. We show directly that FixJ is a transcriptional activator, working in conjunction with the RNA polymerase sigma 70 holoenzyme. Addition of FixL122, a soluble form of the sensor FixL protein, to the transcription assay enhanced FixJ transcriptional activity in response to low oxygen concentration. This enhancement of FixJ activity was correlated with FixJ phosphorylation.

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Year:  1993        PMID： 8226629      PMCID： PMC206811          DOI： 10.1128/jb.175.21.6867-6872.1993

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  28 in total

1.  The FixL protein of Rhizobium meliloti can be separated into a heme-binding oxygen-sensing domain and a functional C-terminal kinase domain.

Authors:  E K Monson; M Weinstein; G S Ditta; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-15       Impact factor: 11.205

2.  Phosphorylation of bacterial response regulator proteins by low molecular weight phospho-donors.

Authors:  G S Lukat; W R McCleary; A M Stock; J B Stock
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-15       Impact factor: 11.205

3.  A haemoprotein with kinase activity encoded by the oxygen sensor of Rhizobium meliloti.

Authors:  M A Gilles-Gonzalez; G S Ditta; D R Helinski
Journal:  Nature       Date:  1991-03-14       Impact factor: 49.962

4.  Modular structure of FixJ: homology of the transcriptional activator domain with the -35 binding domain of sigma factors.

Authors:  D Kahn; G Ditta
Journal:  Mol Microbiol       Date:  1991-04       Impact factor: 3.501

5.  In vitro activity of the nitrogen fixation regulatory protein FIXJ from Rhizobium meliloti.

Authors:  J Batut; E Santero; S Kustu
Journal:  J Bacteriol       Date:  1991-09       Impact factor: 3.490

Review 6.  Signal transduction pathways involving protein phosphorylation in prokaryotes.

Authors:  R B Bourret; K A Borkovich; M I Simon
Journal:  Annu Rev Biochem       Date:  1991       Impact factor: 23.643

7.  Three promoters near the termini of IS10: pIN, pOUT, and pIII.

Authors:  R W Simons; B C Hoopes; W R McClure; N Kleckner
Journal:  Cell       Date:  1983-09       Impact factor: 41.582

8.  Defining a bacteriophage T4 late promoter: absence of a "-35" region.

Authors:  T Elliott; E P Geiduschek
Journal:  Cell       Date:  1984-01       Impact factor: 41.582

9.  Oxygen regulation of nifA transcription in vitro.

Authors:  P G Agron; G S Ditta; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-15       Impact factor: 11.205

10.  The nifA gene of Rhizobium meliloti is oxygen regulated.

Authors:  G Ditta; E Virts; A Palomares; C H Kim
Journal:  J Bacteriol       Date:  1987-07       Impact factor: 3.490
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  22 in total

1.  NodV and NodW, a second flavonoid recognition system regulating nod gene expression in Bradyrhizobium japonicum.

Authors:  J Loh; M Garcia; G Stacey
Journal:  J Bacteriol       Date:  1997-05       Impact factor: 3.490

2.  Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli.

Authors:  Kazushi Suzuki; Xin Wang; Thomas Weilbacher; Anna-Karin Pernestig; Ojar Melefors; Dimitris Georgellis; Paul Babitzke; Tony Romeo
Journal:  J Bacteriol       Date:  2002-09       Impact factor: 3.490

3.  Evidence against the physiological role of acetyl phosphate in the phosphorylation of the ArcA response regulator in Escherichia coli.

Authors:  Xueqiao Liu; Gabriela R Peña Sandoval; Barry L Wanner; Won Seok Jung; Dimitris Georgellis; Ohsuk Kwon
Journal:  J Microbiol       Date:  2009-10-24       Impact factor: 3.422

4.  Structure of a biological oxygen sensor: a new mechanism for heme-driven signal transduction.

Authors:  W Gong; B Hao; S S Mansy; G Gonzalez; M A Gilles-Gonzalez; M K Chan
Journal:  Proc Natl Acad Sci U S A       Date:  1998-12-22       Impact factor: 11.205

5.  Evidence for redox regulation of the transcription factor NtcA, acting both as an activator and a repressor, in the cyanobacterium Anabaena PCC 7120.

Authors:  F Jiang; B Mannervik; B Bergman
Journal:  Biochem J       Date:  1997-10-15       Impact factor: 3.857

6.  Intramolecular signal transduction within the FixJ transcriptional activator: in vitro evidence for the inhibitory effect of the phosphorylatable regulatory domain.

Authors:  S Da Re; S Bertagnoli; J Fourment; J M Reyrat; D Kahn
Journal:  Nucleic Acids Res       Date:  1994-05-11       Impact factor: 16.971

7.  FixJ: a major regulator of the oxygen limitation response and late symbiotic functions of Sinorhizobium meliloti.

Authors:  Christine Bobik; Eliane Meilhoc; Jacques Batut
Journal:  J Bacteriol       Date:  2006-07       Impact factor: 3.490

Review 8.  Genetic regulation of nitrogen fixation in rhizobia.

Authors:  H M Fischer
Journal:  Microbiol Rev       Date:  1994-09

9.  An orphan LuxR homolog of Sinorhizobium meliloti affects stress adaptation and competition for nodulation.

Authors:  Arati V Patankar; Juan E González
Journal:  Appl Environ Microbiol       Date:  2008-12-16       Impact factor: 4.792

10.  Molecular basis of the establishment and functioning of a N2-fixing root nodule.

Authors:  J Michiels; J Vanderleyden
Journal:  World J Microbiol Biotechnol       Date:  1994-11       Impact factor: 3.312
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