BACKGROUND: The clonality of hepatocellular carcinoma is still controversial. To evaluate the clonality of this cancer, mostly small multiple hepatocellular carcinomas of 18 patients were analyzed by DNA fingerprinting. METHODS: DNA from the liver tissues was digested with HinfI. After gel electrophoresis, in gel-hybridization with a synthetic oligonucleotide probe GTGGTGGTGGTGGTG was performed. The band patterns were compared among the tumors and the nontumor part. In patients positive for hepatitis B surface antigen (HBsAg), the integration pattern of hepatitis B viral DNA in liver tissues was also analyzed. RESULTS: Seven of the 9 HBsAg-positive patients had tumors with different clonalities by both methods. In the remaining 9 HBsAg-negative patients, 4 had different band patterns in their tumors by DNA fingerprinting. CONCLUSIONS: Multiple hepatocellular carcinomas frequently have different clonalities; this finding reiterates the importance of eliminating the underlying cause and the contributing factors of hepatocarcinogenesis.
BACKGROUND: The clonality of hepatocellular carcinoma is still controversial. To evaluate the clonality of this cancer, mostly small multiple hepatocellular carcinomas of 18 patients were analyzed by DNA fingerprinting. METHODS: DNA from the liver tissues was digested with HinfI. After gel electrophoresis, in gel-hybridization with a synthetic oligonucleotide probe GTGGTGGTGGTGGTG was performed. The band patterns were compared among the tumors and the nontumor part. In patients positive for hepatitis B surface antigen (HBsAg), the integration pattern of hepatitis B viral DNA in liver tissues was also analyzed. RESULTS: Seven of the 9 HBsAg-positive patients had tumors with different clonalities by both methods. In the remaining 9 HBsAg-negative patients, 4 had different band patterns in their tumors by DNA fingerprinting. CONCLUSIONS:Multiple hepatocellular carcinomas frequently have different clonalities; this finding reiterates the importance of eliminating the underlying cause and the contributing factors of hepatocarcinogenesis.
Authors: Y Sirivatanauksorn; V Sirivatanauksorn; S Bhattacharya; B R Davidson; A P Dhillon; A K Kakkar; R C Williamson; N R Lemoine Journal: Gut Date: 1999-11 Impact factor: 23.059
Authors: Chung Mau Lo; Chi Leung Liu; See Ching Chan; Chi Ming Lam; Ronnie T P Poon; Irene O L Ng; Sheung Tat Fan; John Wong Journal: Ann Surg Date: 2007-06 Impact factor: 12.969
Authors: J C Sheu; Y W Lin; H C Chou; G T Huang; H S Lee; Y H Lin; S Y Huang; C H Chen; J T Wang; P H Lee; J T Lin; F J Lu; D S Chen Journal: Br J Cancer Date: 1999-05 Impact factor: 7.640