Literature DB >> 822421

Determination of molecular weights by fluctuation spectroscopy: application to DNA.

M Weissman, H Schindler, G Feher.   

Abstract

A method for determining molecular weights of macromolecules by measuring spontaneous concentration fluctuations is described. The method is absolute, rapid, and requires no shearing forces on the molecules. We have applied this technique to the determination of molecular weight of DNA molecules. The molecular weight values obtained for T2 phage DNA (1.14 X 10(8)) and replicating Escherichia coli DNA (3.9 X 10(9)) agree with previous results. By monitoring individual molecules, an estimate of the molecular weight of nuclei and individual chromosomal DNA molecules of Drosophila melanogaster was obtained.

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Year:  1976        PMID： 822421      PMCID： PMC430740          DOI： 10.1073/pnas.73.8.2776

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  18 in total

1.  The molecular weights of T2 bacteriophage DNA and its first and second breakage products.

Authors:  I RUBENSTEIN; C A THOMAS; A D HERSHEY
Journal:  Proc Natl Acad Sci U S A       Date:  1961-08       Impact factor: 11.205

2.  Fluctuation spectroscopy: determination of chemical reaction kinetics from the frequency spectrum of fluctuations.

Authors:  G Feher; M Weissman
Journal:  Proc Natl Acad Sci U S A       Date:  1973-03       Impact factor: 11.205

3.  Isolation of membrane-associated folded chromosomes from Escherichia coli: effect of protein synthesis inhibition.

Authors:  O A Ryder; D W Smith
Journal:  J Bacteriol       Date:  1974-12       Impact factor: 3.490

4.  Automated analysis of deoxyribonucleic acid, protein and nuclear to cytoplasmic relationships in tumor cells and gynecologic specimens.

Authors:  J A Steinkamp; H A Crissman
Journal:  J Histochem Cytochem       Date:  1974-07       Impact factor: 2.479

5.  Fluorescence correlation spectroscopy. II. An experimental realization.

Authors:  D Magde; E L Elson; W W Webb
Journal:  Biopolymers       Date:  1974-01       Impact factor: 2.505

6.  Chromosome-sized DNA molecules from Drosophila.

Authors:  R Kavenoff; B H Zimm
Journal:  Chromosoma       Date:  1973       Impact factor: 4.316

7.  Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M.

Authors:  D Freifelder
Journal:  J Mol Biol       Date:  1970-12-28       Impact factor: 5.469

8.  Viscosity and sedimentation of the DNA from bacteriophages T2 and T7 and the relation to molecular weight.

Authors:  D M Crothers; B H Zimm
Journal:  J Mol Biol       Date:  1965-07       Impact factor: 5.469

9.  Complex formation between ethidium bromide and nucleic acids.

Authors:  M J Waring
Journal:  J Mol Biol       Date:  1965-08       Impact factor: 5.469

Review 10.  Physical studies on DNA from "primitive" eucaryotes.

Authors:  T M Roberts; G D Lauer; L C Klotz
Journal:  CRC Crit Rev Biochem       Date:  1975
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  31 in total

1.  Resolving heterogeneity on the single molecular level with the photon-counting histogram.

Authors:  J D Müller; Y Chen; E Gratton
Journal:  Biophys J       Date:  2000-01       Impact factor: 4.033

2.  Dissecting chromatin interactions in living cells from protein mobility maps.

Authors:  Fabian Erdel; Katharina Müller-Ott; Michael Baum; Malte Wachsmuth; Karsten Rippe
Journal:  Chromosome Res       Date:  2011-01       Impact factor: 5.239

3.  Position-sensitive scanning fluorescence correlation spectroscopy.

Authors:  Joseph P Skinner; Yan Chen; Joachim D Müller
Journal:  Biophys J       Date:  2005-05-13       Impact factor: 4.033

4.  Studying slow membrane dynamics with continuous wave scanning fluorescence correlation spectroscopy.

Authors:  Jonas Ries; Petra Schwille
Journal:  Biophys J       Date:  2006-06-16       Impact factor: 4.033

5.  Precise measurement of diffusion coefficients using scanning fluorescence correlation spectroscopy.

Authors:  Zdenek Petrásek; Petra Schwille
Journal:  Biophys J       Date:  2007-10-12       Impact factor: 4.033

6.  Modulated fluorescence correlation spectroscopy with complete time range information.

Authors:  Gustav Persson; Per Thyberg; Jerker Widengren
Journal:  Biophys J       Date:  2007-09-21       Impact factor: 4.033

7.  Separation of the rotational contribution in fluorescence correlation experiments.

Authors:  P Kask; P Piksarv; M Pooga; U Mets; E Lippmaa
Journal:  Biophys J       Date:  1989-02       Impact factor: 4.033

8.  Simplified confocal microscope for counting particles at low concentrations.

Authors:  Joseph P Skinner; Kerry M Swift; Qiaoqiao Ruan; Sergio Perfetto; Enrico Gratton; Sergey Y Tetin
Journal:  Rev Sci Instrum       Date:  2013-07       Impact factor: 1.523

9.  Distribution of molecular aggregation by analysis of fluctuation moments.

Authors:  H Qian; E L Elson
Journal:  Proc Natl Acad Sci U S A       Date:  1990-07       Impact factor: 11.205

10.  Spatial-temporal studies of membrane dynamics: scanning fluorescence correlation spectroscopy (SFCS).

Authors:  Qiaoqiao Ruan; Melanie A Cheng; Moshe Levi; Enrico Gratton; William W Mantulin
Journal:  Biophys J       Date:  2004-08       Impact factor: 4.033
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