Literature DB >> 8223665

Evaluation of a commercial latex agglutination test for rapid detection of Salmonella in fecal samples.

J R Bänffer1, J A van Zwol-Saarloos, L J Broere.   

Abstract

A latex agglutination test for the detection of salmonella in feces was evaluated in comparison to direct culture and enriched culture using both artificially inoculated samples and clinical samples. In the samples inoculated artificially with different concentrations of salmonella (10(1) to 10(5) per gram) the enriched culture performed better only at the 10(2) level in 0.4 g samples, whereas the latex test performed as well as the enriched culture at all levels in 4 g samples. In the tests using clinical samples, there was no significant difference between results of the latex test performed in 2283 samples and the enriched culture performed in 2072 samples. The sensitivity, specificity and negative and positive predictive values of the latex test were 88.2%, 98%, 97.5% and 63% respectively. The test provided results rapidly but yielded a number of false positive results.

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Year:  1993        PMID: 8223665     DOI: 10.1007/bf01973646

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN: 0934-9723            Impact factor:   3.267


  6 in total

Review 1.  New strategies in microbiological diagnosis.

Authors:  M J Pallen; P D Butcher
Journal:  J Hosp Infect       Date:  1991-06       Impact factor: 3.926

2.  Detection of pathogenic Yersinia enterocolitica by the polymerase chain reaction.

Authors:  B W Wren; S Tabaqchali
Journal:  Lancet       Date:  1990-09-15       Impact factor: 79.321

3.  Multi-gene amplification: simultaneous detection of three virulence genes in diarrhoeal stool.

Authors:  G Frankel; J A Giron; J Valmassoi; G K Schoolnik
Journal:  Mol Microbiol       Date:  1989-12       Impact factor: 3.501

Review 4.  Applications of DNA probes for Vero cytotoxin-producing Escherichia coli.

Authors:  H R Smith; G A Willshaw; A Thomas; B Rowe
Journal:  J Hosp Infect       Date:  1991-06       Impact factor: 3.926

5.  Detection of Shigella in feces using DNA amplification.

Authors:  G Frankel; L Riley; J A Giron; J Valmassoi; A Friedmann; N Strockbine; S Falkow; G K Schoolnik
Journal:  J Infect Dis       Date:  1990-06       Impact factor: 5.226

6.  Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction.

Authors:  H Karch; T Meyer
Journal:  J Clin Microbiol       Date:  1989-12       Impact factor: 5.948

  6 in total
  1 in total

Review 1.  Laboratory approaches to infectious diarrhea.

Authors:  D K Turgeon; T R Fritsche
Journal:  Gastroenterol Clin North Am       Date:  2001-09       Impact factor: 3.806

  1 in total

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