Literature DB >> 8223658

Western blot analysis of antibody responses of young infants to pertussis infection.

N Guiso1, E Grimprel, I Anjak, P Bégué.   

Abstract

Western blot and agglutination techniques were used to analyze the antibody responses to Bordetella pertussis in 27 infants less than six month of age with presumed pertussis infection. The antibody response to the Bordetella pertussis adhesions filamentous hemagglutinin, pertactin and agglutinogens, and to the Bordetella pertussis toxins pertussis toxin and adenylate cyclase-hemolysin were compared. Infection induced intense antibody responses to filamentous hemagglutinin, pertussis toxin and adenylate cyclase-hemolysin. Antibodies to agglutinogens were never detected, and antibodies to pertactin were rarely detected in infected infants' sera. Therefore, determination of anti-agglutinogens levels only is not suitable for the serological diagnosis of pertussis in young infants. Use of purified filamentous hemagglutinin, pertussis toxin and adenylate cyclase-hemolysin in Western blot analysis may improve the serodiagnosis of Bordetella infections. However, care must be exercised in distinguishing between the antibody response in young infants and maternally derived antibodies.

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Year:  1993        PMID： 8223658     DOI： 10.1007/bf01973637

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  13 in total

1.  Determination of circulating antibodies directed to pertussis toxin and of agglutinogens in children vaccinated with either the whole cell or component pertussis vaccine in France, Japan and Senegal.

Authors:  E Relyveld; N H Oato; N Guérin; P Coursaget; M Huet; R K Gupta
Journal:  Vaccine       Date:  1991-11       Impact factor: 3.641

Review 2.  Pertussis toxin: the cause of the harmful effects and prolonged immunity of whooping cough. A hypothesis.

Authors:  M Pittman
Journal:  Rev Infect Dis       Date:  1979 May-Jun

3.  Serum IgG, IgA, and IgM responses to pertussis toxin, filamentous hemagglutinin, and agglutinogens 2 and 3 after infection with Bordetella pertussis and immunization with whole-cell pertussis vaccine.

Authors:  M G Thomas; L A Ashworth; E Miller; H P Lambert
Journal:  J Infect Dis       Date:  1989-11       Impact factor: 5.226

4.  Bordetella parapertussis and Bordetella bronchiseptica contain transcriptionally silent pertussis toxin genes.

Authors:  B Aricò; R Rappuoli
Journal:  J Bacteriol       Date:  1987-06       Impact factor: 3.490

5.  Human serum antibody responses to Bordetella pertussis infection and pertussis vaccination.

Authors:  M G Thomas; K Redhead; H P Lambert
Journal:  J Infect Dis       Date:  1989-02       Impact factor: 5.226

6.  Protective activity of Bordetella adenylate cyclase-hemolysin against bacterial colonization.

Authors:  N Guiso; M Szatanik; M Rocancourt
Journal:  Microb Pathog       Date:  1991-12       Impact factor: 3.738

7.  Human serologic response to envelope-associated proteins and adenylate cyclase toxin of Bordetella pertussis.

Authors:  J L Arciniega; E L Hewlett; F D Johnson; A Deforest; S G Wassilak; I M Onorato; C R Manclark; D L Burns
Journal:  J Infect Dis       Date:  1991-01       Impact factor: 5.226

8.  Pertactin, an Arg-Gly-Asp-containing Bordetella pertussis surface protein that promotes adherence of mammalian cells.

Authors:  E Leininger; M Roberts; J G Kenimer; I G Charles; N Fairweather; P Novotny; M J Brennan
Journal:  Proc Natl Acad Sci U S A       Date:  1991-01-15       Impact factor: 11.205

9.  Antibodies to Bordetella pertussis adenylate cyclase are produced in man during pertussis infection and after vaccination.

Authors:  Z Farfel; S Könen; E Wiertz; R Klapmuts; P A Addy; E Hanski
Journal:  J Med Microbiol       Date:  1990-07       Impact factor: 2.472

10.  Characterization of the protective capacity and immunogenicity of the 69-kD outer membrane protein of Bordetella pertussis.

Authors:  R D Shahin; M J Brennan; Z M Li; B D Meade; C R Manclark
Journal:  J Exp Med       Date:  1990-01-01       Impact factor: 14.307
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  8 in total

1.  Polymorphism of Bordetella pertussis isolates circulating for the last 10 years in France, where a single effective whole-cell vaccine has been used for more than 30 years.

Authors:  C Weber; C Boursaux-Eude; G Coralie; V Caro; N Guiso
Journal:  J Clin Microbiol       Date:  2001-12       Impact factor: 5.948

Review 2.  Laboratory diagnosis of pertussis: state of the art in 1997.

Authors:  F M Müller; J E Hoppe; C H Wirsing von König
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

3.  Long-term human serum antibody responses after immunization with whole-cell pertussis vaccine in France.

Authors:  E Grimprel; P Bégué; I Anjak; E Njamkepo; P François; N Guiso
Journal:  Clin Diagn Lab Immunol       Date:  1996-01

Review 4.  Bordetella Pertussis virulence factors in the continuing evolution of whooping cough vaccines for improved performance.

Authors:  Dorji Dorji; Frits Mooi; Osvaldo Yantorno; Rajendar Deora; Ross M Graham; Trilochan K Mukkur
Journal:  Med Microbiol Immunol       Date:  2017-11-21       Impact factor: 3.402

Review 5.  Laboratory Diagnosis of Pertussis.

Authors:  Anneke van der Zee; Joop F P Schellekens; Frits R Mooi
Journal:  Clin Microbiol Rev       Date:  2015-10       Impact factor: 26.132

6.  Rapid diagnosis of pertussis in young infants: comparison of culture, PCR, and infant's and mother's serology.

Authors:  E Grimprel; E Njamkepo; P Bégué; N Guiso
Journal:  Clin Diagn Lab Immunol       Date:  1997-11

7.  Comparison of commercially available immunoblot assays measuring IgG and IgA antibodies to Bordetella pertussis antigens.

Authors:  N Kennerknecht; M Riffelmann; J Schmetz; C H Wirsing von König
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-04-20       Impact factor: 3.267

8.  Pertussis surveillance in private pediatric practices, France, 2002-2006.

Authors:  Nicole Guiso; France de La Rocque; Elisabeth Njamkepo; Aurelie Lécuyer; Corinne Levy; Olivier Romain; Franck Thollot; Véronique Abitbol; Benoit Soubeyrand; Robert Cohen
Journal:  Emerg Infect Dis       Date:  2008-07       Impact factor: 6.883
  8 in total

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