Literature DB >> 8222753

Increased FISH efficiency using APC probes generated by direct incorporation of labeled nucleotides by PCR.

F Richard1, N Vogt, M Muleris, B Malfoy, B Dutrillaux.   

Abstract

Probes of various sizes from the adenomatous polyposis coli gene (APC) were directly biotinylated by polymerase chain reaction (PCR) from genomic DNA. PCR labeling gave high efficiency in detection of fluorescence in situ hybridization (FISH) signals. Probes as small as 250 base pairs could be visualized through a fluorescence microscope without any image processing.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 8222753     DOI： 10.1159/000133624

Source DB:  PubMed          Journal:  Cytogenet Cell Genet        ISSN： 0301-0171


  8 in total

1.  Mapping ESTs by fiber-FISH.

Authors:  N Horelli-Kuitunen; J Aaltonen; M L Yaspo; M Eeva; M Wessman; L Peltonen; A Palotie
Journal:  Genome Res       Date:  1999-01       Impact factor: 9.043

2.  Skewed inactivation of an X chromosome deleted at the dystrophin gene in an asymptomatic mother and her affected daughter.

Authors:  F Tihy; N Vogt; D Recan; B Malfoy; F Leturcq; M Coquet; F Serville; D Fontan; J M Guillard; J C Kaplan
Journal:  Hum Genet       Date:  1994-05       Impact factor: 4.132

3.  A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods.

Authors:  B D Cobb; J M Clarkson
Journal:  Nucleic Acids Res       Date:  1994-09-11       Impact factor: 16.971

4.  FISH mapping of seven cDNA sequences in the pig.

Authors:  R Chaudhary; A K Winterø; M Fredholm; B P Chowdhary
Journal:  Chromosome Res       Date:  1997-12       Impact factor: 5.239

5.  Scanning ion analytical microscopy for high-resolution detection of 5-bromo-2'-deoxyuridine incorporation in metaphase chromosomes.

Authors:  C A Bourgeois; R Dennebouy; A Gibaud; M Gerbault-Seureau; B Malfoy; G Slodzian; P Galle; B Dutrillaux
Journal:  Chromosome Res       Date:  1996-12       Impact factor: 5.239

6.  Malignant conversion of chemically transformed normal human cells.

Authors:  G E Milo; D Li; B C Casto; K Theil; C Shuler; I Noyes; J Chen
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-28       Impact factor: 11.205

7.  Location of low copy genes in chromosomes of Brachiaria spp.

Authors:  Thaís Furtado Nani; James C Schnable; Jacob D Washburn; Patrice Albert; Welison Andrade Pereira; Fausto Souza Sobrinho; James A Birchler; Vânia Helena Techio
Journal:  Mol Biol Rep       Date:  2018-01-12       Impact factor: 2.316

8.  Localization of a female-specific marker on the chromosomes of the brown seaweed Saccharina japonica using fluorescence in situ hybridization.

Authors:  Yu Liu; YanHui Bi; JunGang Gu; LiHua Li; ZhiGang Zhou
Journal:  PLoS One       Date:  2012-11-07       Impact factor: 3.240
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.