| Literature DB >> 8220456 |
V I Klimyuk1, B J Carroll, C M Thomas, J D Jones.
Abstract
For plant genetics, it would be useful to monitor easily the segregation of different alleles using the polymerase chain reaction (PCR). Preparation of DNA templates from individual plants needs to be rapid and reliable. A one tube protocol is described that involves subjecting plant tissue pieces to alkali, neutralization and heat denaturation prior to PCR analysis, and that proved to be much faster and more reliable than published protocols.Mesh：
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Year: 1993 PMID： 8220456 DOI： 10.1111/j.1365-313x.1993.tb00169.x
Source DB: PubMed Journal: Plant J ISSN： 0960-7412 Impact factor: 6.417