| Literature DB >> 8220190 |
Abstract
Anomalous PCR products are often produced during the amplification of d(CA)n.d(TG)n sequences. Upon denaturing polyacrylamide gel electrophoresis, these products yield a ladder-like pattern that can complicate genotypic interpretation. We have developed two related techniques, referred to as two- and three-stage linear amplification (2-SLA and 3-SLA, respectively), which largely overcome this problem and yield readily interpretable banding patterns.Entities:
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Year: 1993 PMID： 8220190 DOI： 10.1101/gr.3.1.7
Source DB: PubMed Journal: PCR Methods Appl ISSN： 1054-9803