Literature DB >> 8217345

A rapid, automated microtiter assay for measuring neutralization of HIV-1.

S Laal1, S Burda, S Sharpe, S Zolla-Pazner.   

Abstract

A sensitive, rapid, and quantitative ELISA for p24 is described, which can be used as the read-out to test for HIV-1 neutralization in the syncytium-forming microassay and can replace the counting of syncytia under the microscope. This assay can be used reliably for divergent strains of HIV-1, including those that do not induce syncytia. The new read-out permits evaluation of neutralization at 3 days rather than 5 days. Using this assay, the neutralizing activity of several new and previously described human monoclonal antibodies against HIV-1 was characterized.

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Year:  1993        PMID： 8217345     DOI： 10.1089/aid.1993.9.781

Source DB:  PubMed          Journal:  AIDS Res Hum Retroviruses        ISSN： 0889-2229            Impact factor:   2.205


  5 in total

1.  Infection by discordant strains of HIV-1 markedly enhances the neutralizing antibody response against heterologous virus.

Authors:  Rebecca L R Powell; Thompson Kinge; Phillipe N Nyambi
Journal:  J Virol       Date:  2010-07-14       Impact factor: 5.103

2.  Neutralization profiles of primary human immunodeficiency virus type 1 isolates in the context of coreceptor usage.

Authors:  D Cecilia; V N KewalRamani; J O'Leary; B Volsky; P Nyambi; S Burda; S Xu; D R Littman; S Zolla-Pazner
Journal:  J Virol       Date:  1998-09       Impact factor: 5.103

3.  Neutralization of primary human immunodeficiency virus type 1 isolates by the broadly reactive anti-V3 monoclonal antibody, 447-52D.

Authors:  A J Conley; M K Gorny; J A Kessler; L J Boots; M Ossorio-Castro; S Koenig; D W Lineberger; E A Emini; C Williams; S Zolla-Pazner
Journal:  J Virol       Date:  1994-11       Impact factor: 5.103

4.  Synergistic neutralization of human immunodeficiency virus type 1 by combinations of human monoclonal antibodies.

Authors:  S Laal; S Burda; M K Gorny; S Karwowska; A Buchbinder; S Zolla-Pazner
Journal:  J Virol       Date:  1994-06       Impact factor: 5.103

5.  Human anti-V2 monoclonal antibody that neutralizes primary but not laboratory isolates of human immunodeficiency virus type 1.

Authors:  M K Gorny; J P Moore; A J Conley; S Karwowska; J Sodroski; C Williams; S Burda; L J Boots; S Zolla-Pazner
Journal:  J Virol       Date:  1994-12       Impact factor: 5.103
  5 in total

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