| Literature DB >> 8217303 |
K Patel1, D Sheer, G M Hampton.
Abstract
A new and potentially reliable method for the isolation of yeast artificial chromosome (YAC)-insert termini, termed junction trapping, is described. This method is based on simple partial digestions of the YAC-containing yeast strain, ligation to a plasmid vector, and selection of the termini by two rounds of polymerase chain reaction (PCR). To date, the method has generated both terminal sequences from each of nine YACs (100%) that have been examined.Entities:
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Year: 1993 PMID： 8217303 DOI： 10.1016/1050-3862(93)90044-j
Source DB: PubMed Journal: Genet Anal Tech Appl ISSN： 1050-3862