Literature DB >> 8216768

Cryopreservation of cultured dermal fibroblast impregnated collagen gels.

B Teasdale1, V K Sieber, D J Riches, J Nanchahal.   

Abstract

The survival of cultured dermal fibroblasts was evaluated following manufacture, freezing and disaggregation of fibroblast-impregnated collagen gels. The concentration which gave optimal cell survival was determined for three cryoprotectants (glycerol, dimethyl, sulphoxide (DMSO) and ethanediol) and their efficacy compared. DMSO led to the highest cell viability after freezing and thawing. The effect of rate of freezing was also compared and 0.5 degree C/min (within the range 20 degrees C to -70 degrees C) was found to result in a significant enhancement of cell viability in comparison with freezing at 1.0 degree C/min or rapid freezing.

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Year:  1993        PMID： 8216768     DOI： 10.1016/0305-4179(93)90062-d

Source DB:  PubMed          Journal:  Burns        ISSN： 0305-4179            Impact factor:   2.744


  2 in total

1.  Distinct Lipidomic Landscapes Associated with Clinical Stages of Urothelial Cancer of the Bladder.

Authors:  Danthasinghe Waduge Badrajee Piyarathna; Thekkelnaycke M Rajendiran; Vasanta Putluri; Venkatrao Vantaku; Tanu Soni; Friedrich-Carl von Rundstedt; Sri Ramya Donepudi; Feng Jin; Suman Maity; Chandrashekar R Ambati; Jianrong Dong; Daniel Gödde; Stephan Roth; Stephan Störkel; Stephan Degener; George Michailidis; Seth P Lerner; Subramaniam Pennathur; Yair Lotan; Cristian Coarfa; Arun Sreekumar; Nagireddy Putluri
Journal:  Eur Urol Focus       Date:  2017-04-20

2.  Effect of different freezing rates during cryopreservation of rat mesenchymal stem cells using combinations of hydroxyethyl starch and dimethylsulfoxide.

Authors:  Yahaira Naaldijk; Marek Staude; Viktoriya Fedorova; Alexandra Stolzing
Journal:  BMC Biotechnol       Date:  2012-08-13       Impact factor: 2.563
  2 in total

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