Literature DB >> 8215384

Evaluation of radioactive and nonradioactive gene probes and cell culture for detection of poliovirus in water samples.

N J Moore1, A B Margolin.   

Abstract

Five nonradioactive probe assays were evaluated by using chemiluminescent and colormetric signals, along with two isotopic assays and cell culture, for the detection of poliovirus in concentrated water samples. In environmental samples, a 100% correlation existed between digoxigenin and single-stranded [32P]RNA probes. All probe assays detected more positive samples than the cell culture did.

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Year:  1993        PMID： 8215384      PMCID： PMC182422          DOI： 10.1128/aem.59.9.3145-3146.1993

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  4 in total

1.  Organic flocculation: an efficient second-step concentration method for the detection of viruses in tap water.

Authors:  E Katzenelson; B Fattal; T Hostovesky
Journal:  Appl Environ Microbiol       Date:  1976-10       Impact factor: 4.792

2.  Rotavirus in travelers' diarrhea: study of an adult student population in Mexico.

Authors:  R Bolivar; R H Conklin; J J Vollet; L K Pickering; H L DuPont; D L Walters; S Kohl
Journal:  J Infect Dis       Date:  1978-03       Impact factor: 5.226

3.  A novel method for liberating viral nucleic acid for assay of water samples with cDNA probes.

Authors:  K J Richardson; A B Margolin; C P Gerba
Journal:  J Virol Methods       Date:  1988-10       Impact factor: 2.014

4.  Optimization of the BGM cell line culture and viral assay procedures for monitoring viruses in the environment.

Authors:  D R Dahling; B A Wright
Journal:  Appl Environ Microbiol       Date:  1986-04       Impact factor: 4.792
  4 in total
  2 in total

1.  Detection of enteroviruses and hepatitis A virus in water by consensus primer multiplex RT-PCR.

Authors:  Jun-Wen Li; Xin-Wei Wang; Chang-Qing Yuan; Jin-Lai Zheng; Min Jin; Nong Song; Xiu-Quan Shi; Fu-Huan Chao
Journal:  World J Gastroenterol       Date:  2002-08       Impact factor: 5.742

2.  Efficacy of Nucleic Acid Probes for Detection of Poliovirus in Water Disinfected by Chlorine, Chlorine Dioxide, Ozone, and UV Radiation.

Authors:  N J Moore; A B Margolin
Journal:  Appl Environ Microbiol       Date:  1994-11       Impact factor: 4.792
  2 in total

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