Literature DB >> 8215382

Gene cloning, sequencing, and biochemical characterization of endoxylanase from Thermoanaerobacterium saccharolyticum B6A-RI.

Y E Lee1, S E Lowe, J G Zeikus.   

Abstract

The gene encoding endoxylanase (xynA) from Thermoanaerobacterium saccharolyticum B6A-RI was cloned and expressed in Escherichia coli. A putative 33-amino-acid signal peptide, which corresponded to the N-terminal amino acids, was encoded by xynA. An open reading frame of 3,471 bp, corresponding to 1,157 amino acid residues, was found, giving the xynA gene product a molecular mass of 130 kDa. xynA from T. saccharolyticum B6A-RI had strong similarity to genes from family F beta-glycanases. The temperature and pH optimum for the activity of the cloned endoxylanase were 70 degrees C and 5.5, respectively. The cloned endoxylanase A was stable at 75 degrees C for 60 min and displayed a specific activity of 227.4 U/mg of protein on oat spelt xylan. The cloned xylanase was an endo-acting enzyme.

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Year:  1993        PMID： 8215382      PMCID： PMC182419          DOI： 10.1128/aem.59.9.3134-3137.1993

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  Studies on xylan degrading enzymes. I. Purification and characterization of endo-1,4-beta-xylanase from Aspergillus niger str. 14.

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Journal:  Biochim Biophys Acta       Date:  1977-09-15

Review 2.  Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.

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Journal:  Microbiol Rev       Date:  1991-06

3.  Differences in Xylan Degradation by Various Noncellulolytic Thermophilic Anaerobes and Clostridium thermocellum.

Authors:  J Wiegel; C P Mothershed; J Puls
Journal:  Appl Environ Microbiol       Date:  1985-03       Impact factor: 4.792

4.  Conserved serine-rich sequences in xylanase and cellulase from Pseudomonas fluorescens subspecies cellulosa: internal signal sequence and unusual protein processing.

Authors:  J Hall; G P Hazlewood; N S Huskisson; A J Durrant; H J Gilbert
Journal:  Mol Microbiol       Date:  1989-09       Impact factor: 3.501

5.  Molecular cloning of genes from Ruminococcus flavefaciens encoding xylanase and beta(1-3,1-4)glucanase activities.

Authors:  H J Flint; C A McPherson; J Bisset
Journal:  Appl Environ Microbiol       Date:  1989-05       Impact factor: 4.792

6.  Relationship of cellulosomal and noncellulosomal xylanases of Clostridium thermocellum to cellulose-degrading enzymes.

Authors:  E Morag; E A Bayer; R Lamed
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

7.  Purification of Clostridium thermocellum xylanase Z expressed in Escherichia coli and identification of the corresponding product in the culture medium of C. thermocellum.

Authors:  O Grépinet; M C Chebrou; P Béguin
Journal:  J Bacteriol       Date:  1988-10       Impact factor: 3.490

8.  Thermophilic anaerobic bacteria which ferment hemicellulose: characterization of organisms and identification of plasmids.

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Journal:  Arch Microbiol       Date:  1984-05       Impact factor: 2.552

9.  Cloning of a xylanase gene from Fibrobacter succinogenes 135 and its expression in Escherichia coli.

Authors:  Y J Hu; D C Smith; K J Cheng; C W Foresberg
Journal:  Can J Microbiol       Date:  1991-07       Impact factor: 2.419

10.  Xylan-degrading enzymes of the yeast Cryptococcus albidus. Identification and cellular localization.

Authors:  P Biely; M Vrsanská; Z Krátký
Journal:  Eur J Biochem       Date:  1980
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  15 in total

Review 1.  Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability.

Authors:  C Vieille; G J Zeikus
Journal:  Microbiol Mol Biol Rev       Date:  2001-03       Impact factor: 11.056

2.  Cell surface xylanases of the glycoside hydrolase family 10 are essential for xylan utilization by Paenibacillus sp. W-61 as generators of xylo-oligosaccharide inducers for the xylanase genes.

Authors:  Mutsumi Fukuda; Seiji Watanabe; Shigeki Yoshida; Hiroya Itoh; Yoshifumi Itoh; Yoshiyuki Kamio; Jun Kaneko
Journal:  J Bacteriol       Date:  2010-02-12       Impact factor: 3.490

3.  Cloning of the xynB gene from Dictyoglomus thermophilum Rt46B.1 and action of the gene product on kraft pulp.

Authors:  D D Morris; M D Gibbs; C W Chin; M H Koh; K K Wong; R W Allison; P J Nelson; P L Bergquist
Journal:  Appl Environ Microbiol       Date:  1998-05       Impact factor: 4.792

4.  Cloning, sequencing, and expression of the gene encoding a large S-layer-associated endoxylanase from Thermoanaerobacterium sp. strain JW/SL-YS 485 in Escherichia coli.

Authors:  S Y Liu; F C Gherardini; M Matuschek; H Bahl; J Wiegel
Journal:  J Bacteriol       Date:  1996-03       Impact factor: 3.490

5.  Profile of secreted hydrolases, associated proteins, and SlpA in Thermoanaerobacterium saccharolyticum during the degradation of hemicellulose.

Authors:  D H Currie; A M Guss; C D Herring; R J Giannone; C M Johnson; P K Lankford; S D Brown; R L Hettich; L R Lynd
Journal:  Appl Environ Microbiol       Date:  2014-06-06       Impact factor: 4.792

6.  Isolation and expression of the xynB gene and its product, XynB, a consistent component of the Clostridium cellulovorans cellulosome.

Authors:  Sung Ok Han; Hideaki Yukawa; Masayuki Inui; Roy H Doi
Journal:  J Bacteriol       Date:  2004-12       Impact factor: 3.490

7.  A High-Molecular-Weight, Cell-Associated Xylanase Isolated from Exponentially Growing Thermoanaerobacterium sp. Strain JW/SL-YS485.

Authors:  W Shao; S Deblois; J Wiegel
Journal:  Appl Environ Microbiol       Date:  1995-03       Impact factor: 4.792

8.  Cloning, DNA sequencing, and expression of the gene encoding Clostridium thermocellum cellulase CelJ, the largest catalytic component of the cellulosome.

Authors:  M M Ahsan; T Kimura; S Karita; K Sakka; K Ohmiya
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

9.  Influence of the transposition of the thermostabilizing domain of Clostridium thermocellum xylanase (XynX) on xylan binding and thermostabilization.

Authors:  Eun-Sun Shin; Mi-Jeong Yang; Kyung Hwa Jung; Eun-Ju Kwon; Jae Sung Jung; Seur Kee Park; Jungho Kim; Han Dae Yun; Hoon Kim
Journal:  Appl Environ Microbiol       Date:  2002-07       Impact factor: 4.792

10.  Characterization of the active site and thermostability regions of endoxylanase from Thermoanaerobacterium saccharolyticum B6A-RI.

Authors:  Y E Lee; S E Lowe; B Henrissat; J G Zeikus
Journal:  J Bacteriol       Date:  1993-09       Impact factor: 3.490
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