| Literature DB >> 8210675 |
A A Vertès1, M Inui, M Kobayashi, Y Kurusu, H Yukawa.
Abstract
Efficient transformation of Brevibacterium flavum MJ233C and Corynebacterium glutamicum ATCC 31831 (up to 5.0 x 10(7) transformants/microgram DNA) depends on the source of plasmid DNA. The transformation efficiencies of B. flavum MJ233C and C. glutamicum ATCC 31831 increased nearly 10(3)-fold when plasmid DNA was isolated from the recipient strain itself or from a damdcm Escherichia coli mutant, as compared with DNA passed through a modification-proficient E. coli strain. These results suggest the presence of a methyl-specific restriction system in certain strains of coryneform bacteria. In addition, electroporation conditions were optimized.Entities:
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Year: 1993 PMID: 8210675 DOI: 10.1016/0923-2508(93)90043-2
Source DB: PubMed Journal: Res Microbiol ISSN: 0923-2508 Impact factor: 3.992