| Literature DB >> 8209735 |
E J Snijder1, A L Wassenaar, W J Spaan.
Abstract
A papainlike cysteine protease (PCP) domain in the N-terminal region of the equine arteritis virus (EAV) replicase was identified by in vitro translation and mutagenesis studies. The EAV protease was found to direct an autoproteolytic cleavage at its C-terminus which leads to the production of an approximately 30K N-terminal replicase product (nsp1) containing the PCP domain. Amino acid residues Cys164 and His230 of the EAV replicase polyprotein were identified as the most likely candidates for the role of PCP catalytic residues. It was shown that cleavage occurs in cis between Gly260 and Gly261.Entities:
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Year: 1993 PMID： 8209735 DOI： 10.1007/978-1-4615-2996-5_36
Source DB: PubMed Journal: Adv Exp Med Biol ISSN： 0065-2598 Impact factor: 2.622