B V Magno1, V Freidlin, M B Datiles. 1. Ophthalmic Genetics and Clinical Services Branch, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892.
Abstract
PURPOSE: The NEI Scheimpflug Cataract Imaging System was developed to allow for easy, accurate and reproducible image analysis of nuclear cataracts. This study was undertaken to determine the reproducibility of densitometric measurements of the lens nucleus using this modified system. METHODS: Replicate Zeiss Scheimpflug images of the lenses in 143 eyes were obtained by one photographer. Normal and cataractous lenses (without central cortical or anterior subcapsular opacities) were sampled. Images were stored after testing for adequacy using immediate exposure checking. Densitometry of the nuclear region was then performed for each image. The interval within which 99% of the differences between repeat measurements may be expected to lie was used as a measure of reproducibility (99% range). RESULTS: A 99% range of +/- 0.023 optical density units (odu) was obtained for nuclear densities < 0.30 odu (125 eyes). For lenses with nuclear densities > or = 0.30 odu (18 eyes), the 99% range was +/- 0.14 times the first measurement. CONCLUSION: This study shows the excellent reproducibility of this Scheimpflug imaging system in the nuclear region and demonstrates its usefulness in studies on nuclear cataracts, particularly for natural history studies and clinical trials of anti-cataract drugs.
PURPOSE: The NEI Scheimpflug Cataract Imaging System was developed to allow for easy, accurate and reproducible image analysis of nuclear cataracts. This study was undertaken to determine the reproducibility of densitometric measurements of the lens nucleus using this modified system. METHODS: Replicate Zeiss Scheimpflug images of the lenses in 143 eyes were obtained by one photographer. Normal and cataractous lenses (without central cortical or anterior subcapsular opacities) were sampled. Images were stored after testing for adequacy using immediate exposure checking. Densitometry of the nuclear region was then performed for each image. The interval within which 99% of the differences between repeat measurements may be expected to lie was used as a measure of reproducibility (99% range). RESULTS: A 99% range of +/- 0.023 optical density units (odu) was obtained for nuclear densities < 0.30 odu (125 eyes). For lenses with nuclear densities > or = 0.30 odu (18 eyes), the 99% range was +/- 0.14 times the first measurement. CONCLUSION: This study shows the excellent reproducibility of this Scheimpflug imaging system in the nuclear region and demonstrates its usefulness in studies on nuclear cataracts, particularly for natural history studies and clinical trials of anti-cataract drugs.
Authors: Ling Bei; Ying-Bo Shui; Fang Bai; Suzanne K Nelson; Gregory P Van Stavern; David C Beebe Journal: Exp Eye Res Date: 2015-03-13 Impact factor: 3.467
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