Literature DB >> 8206173

Comparison of methods used for detection of mycoplasma contamination in cell cultures, sera, and live-virus vaccines.

T Benisheva1, V Sovová, I Ivanov, G Opalchenova.   

Abstract

Two methods for detection of mycoplasma contamination in cell cultures, sera, and live-virus vaccines were compared: the direct culture test and the DNA staining method employing bisBenzimide (Hoechst No. 33258). Contamination by different species of mycoplasma was found in 39% samples tested. It is recommended to use both techniques for a reliable detection of mycoplasma contamination.

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Year:  1993        PMID: 8206173

Source DB:  PubMed          Journal:  Folia Biol (Praha)        ISSN: 0015-5500            Impact factor:   0.906


  2 in total

1.  Species-specific PCR for identification of common contaminant mollicutes in cell culture.

Authors:  F Kong; G James; S Gordon; A Zelynski; G L Gilbert
Journal:  Appl Environ Microbiol       Date:  2001-07       Impact factor: 4.792

2.  Biological enrichment of Mycoplasma agents by cocultivation with permissive cell cultures.

Authors:  Dmitriy V Volokhov; Hyesuk Kong; Joseph George; Christine Anderson; Vladimir E Chizhikov
Journal:  Appl Environ Microbiol       Date:  2008-07-07       Impact factor: 4.792

  2 in total

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