Literature DB >> 820571

Purification of RNA from animal cells using diethyl-pyrocarbonate.

D Tsuei, H Rouhandeh.   

Abstract

Extraction of RNA from animal cells by a method using diethyl-pyrocarbonate yielded 50-60% of the total RNA. RNA purified by a hot phenol-SDS method from adenovirus 2 infected cells showed about 9% homology with adenovirus DNA, and RNA purified by diethyl-pyrocarbonate-SDS showed over 7% hybridization. Profiles of RNA prepared by both methods were identical when studied by polyacrylamide gel electrophoresis.

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Year:  1976        PMID： 820571     DOI： 10.1007/BF01919868

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  11 in total

1.  BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION. VII. HOMOLOGY BETWEEN DNA'S OF TUMORIGENIC AND NONTUMORIGENIC HUMAN ADENOVIRUSES.

Authors:  S LACY; M GREEN
Journal:  Proc Natl Acad Sci U S A       Date:  1964-10       Impact factor: 11.205

2.  Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus.

Authors:  M GREEN; M PINA
Journal:  Virology       Date:  1963-05       Impact factor: 3.616

3.  A simple method for extraction of RNA from E. coli utilizing diethyl pyrocarbonate.

Authors:  W C Summers
Journal:  Anal Biochem       Date:  1970-02       Impact factor: 3.365

4.  Biochemical studies on adenovirus multiplication. 18. Resolution of early virus-specific RNA species in Ad 2 infected and transformed cells.

Authors:  J T Parsons; M Green
Journal:  Virology       Date:  1971-07       Impact factor: 3.616

5.  Mechanism of viral carcinogenesis by DNA mammalian viruses. V. Properties of purified viral-specific RNA from human adenovirus-induced tumor cells.

Authors:  K Fujinaga; M Green
Journal:  J Mol Biol       Date:  1968-01-14       Impact factor: 5.469

6.  The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.

Authors:  U E Loening
Journal:  Biochem J       Date:  1967-01       Impact factor: 3.857

7.  Electrophoretic separation of viral nucleic acids on polyacrylamide gels.

Authors:  D H Bishop; J R Claybrook; S Spiegelman
Journal:  J Mol Biol       Date:  1967-06-28       Impact factor: 5.469

8.  Effects of diethyl pyrocarbonate and methyl methanesulfonate on nucleic acids and nucleases.

Authors:  I Fedorcsák; L Ehrenberg
Journal:  Acta Chem Scand       Date:  1966

9.  Rapidly labeled HeLa cell nuclear RNA. I. Identification by zone sedimentation of a heterogeneous fraction separate from ribosomal precursor RNA.

Authors:  J R Warner; R Soeiro; H C Birnboim; M Girard; J E Darnell
Journal:  J Mol Biol       Date:  1966-08       Impact factor: 5.469

10.  Inhibition of the synthesis of simian virus 40 antigens in cells preinfected with Yaba tumor virus.

Authors:  Y Tsuchiya; H Rouhandeh
Journal:  J Virol       Date:  1971-11       Impact factor: 5.103
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