Literature DB >> 8203122

Cloning and sequencing of the chicken anaemia virus (CAV) ORF-3 gene, and the development of an ELISA for the detection of serum antibody to CAV.

J Pallister1, K J Fahey, M Sheppard.   

Abstract

Chicken anaemia virus (CAV) is a small, unclassified virus involved in anaemia and suspected of causing immunosuppression in young chickens. We have developed an ELISA for the detection of serum antibody to CAV based on cloned antigen. The gene for ORF-3 (the putative capsid protein) was cloned, sequenced and expressed in a bacterial expression system, pGEX. An ORF-3 fusion protein was used to produce an indirect ELISA.

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Year:  1994        PMID： 8203122     DOI： 10.1016/0378-1135(94)90097-3

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN： 0378-1135            Impact factor:   3.293


  7 in total

1.  A hypervariable region in VP1 of chicken infectious anemia virus mediates rate of spread and cell tropism in tissue culture.

Authors:  R W Renshaw; C Soiné; T Weinkle; P H O'Connell; K Ohashi; S Watson; B Lucio; S Harrington; K A Schat
Journal:  J Virol       Date:  1996-12       Impact factor: 5.103

2.  High yield expression in a recombinant E. coli of a codon optimized chicken anemia virus capsid protein VP1 useful for vaccine development.

Authors:  Meng-Shiou Lee; You-Cheng Hseu; Guan-Hua Lai; Wen-Te Chang; Hsi-Jien Chen; Chi-Hung Huang; Meng-Shiunn Lee; Min-Ying Wang; Jung-Yie Kao; Bang-Jau You; Wen- Hsin Lin; Yi-Yang Lien; Ming-Kuem Lin
Journal:  Microb Cell Fact       Date:  2011-07-23       Impact factor: 5.328

3.  Identification of the NLS and NES motifs of VP2 from chicken anemia virus and the interaction of VP2 with mini-chromosome maintenance protein 3.

Authors:  Jai-Hong Cheng; Shyang-Chwen Sheu; Yi-Yang Lien; Meng-Shiunn Lee; His-Jien Chen; Wen-Hong Su; Meng-Shiou Lee
Journal:  BMC Vet Res       Date:  2012-02-07       Impact factor: 2.741

4.  Development and characterization of a potential diagnostic monoclonal antibody against capsid protein VP1 of the chicken anemia virus.

Authors:  Yi-Yang Lien; Chi-Hung Huang; Fang-Chun Sun; Shyang-Chwen Sheu; Tsung-Chi Lu; Meng-Shiunn Lee; Shu-Chin Hsueh; Hsi-Jien Chen; Meng-Shiou Lee
Journal:  J Vet Sci       Date:  2012-03       Impact factor: 1.672

5.  Preparation of Chicken Anemia Virus (CAV) Virus-Like Particles and Chicken Interleukin-12 for Vaccine Development Using a Baculovirus Expression System.

Authors:  Ta-Yuan Tseng; Yee-Chen Liu; Yu-Chen Hsu; Poa-Chun Chang; Ming-Kun Hsieh; Jui-Hung Shien; Shan-Chia Ou
Journal:  Pathogens       Date:  2019-11-23

6.  A new rapid and sensitive method for detecting chicken infectious anemia virus.

Authors:  Xiuhong Wu; Jie Kong; Ziqi Yao; Hejing Sun; Yuanjia Liu; Zhiqiang Wu; Jiajia Liu; Hao Zhang; Haohua Huang; Jin Wang; Mengjun Chen; Yichen Zeng; Yinpeng Huang; Feng Chen; Qingmei Xie; Xinheng Zhang
Journal:  Front Microbiol       Date:  2022-09-29       Impact factor: 6.064

7.  Expression and characterization of highly antigenic domains of chicken anemia virus viral VP2 and VP3 subunit proteins in a recombinant E. coli for sero-diagnostic applications.

Authors:  Guan-Hua Lai; Ming-Kuem Lin; Yi-Yang Lien; Jiun-Hau Fu; Hsi-Jien Chen; Chi-Hung Huang; Jason T C Tzen; Meng-Shiou Lee
Journal:  BMC Vet Res       Date:  2013-08-13       Impact factor: 2.741
  7 in total

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