Literature DB >> 820257

Improved method for preparing anaerobic bacteroid suspensions of Rhizobium leguminosarum for the acetylene reduction assay.

J Van Straten, W Roelofsen.   

Abstract

A method using ethylenediaminetetraacetate (EDTA) and toluene-treated pea bacteroid suspensions for the acetylene reduction assay is described. The high level of acetylene reduction by these bacteroids is comparable to that of intact plants. Reproducibility of the EDTA-toluene treatment is, on the average, within 5%. Preliminary experiments with soybeans indicate that the EDTA-toluene method might be applicable to other legumes as well.

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Year:  1976        PMID： 820257      PMCID： PMC169846          DOI： 10.1128/aem.31.6.859-863.1976

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

1.  ACTINOMYCIN SENSITIVITY IN ESCHERICHIA COLI PRODUCED BY EDTA.

Authors:  L LEIVE
Journal:  Biochem Biophys Res Commun       Date:  1965-01-04       Impact factor: 3.575

2.  A NONSPECIFIC INCREASE IN PERMEABILITY IN ESCHERICHIA COLI PRODUCED BY EDTA.

Authors:  L LEIVE
Journal:  Proc Natl Acad Sci U S A       Date:  1965-04       Impact factor: 11.205

3.  Studies on the induction of beta-galactosidase in a cryptic strain of Escherichia coli.

Authors:  L A HERZENBERG
Journal:  Biochim Biophys Acta       Date:  1959-02

4.  Reactivation and hybridization of reduced alkaline phosphatase.

Authors:  C LEVINTHAL; E R SIGNER; K FETHEROLF
Journal:  Proc Natl Acad Sci U S A       Date:  1962-07-15       Impact factor: 11.205

5.  Estimation of nitrogenase in intact legumes.

Authors:  T A LaRue; W G Kurz
Journal:  Can J Microbiol       Date:  1973-02       Impact factor: 2.419

6.  Studies on the permeability change produced in coliform bacteria by ethylenediaminetetraacetate.

Authors:  L Leive
Journal:  J Biol Chem       Date:  1968-05-10       Impact factor: 5.157

7.  The effect of ethylenediaminetetra-acetic acid on the cell walls of some gram-negative bacteria.

Authors:  G W Gray; S G Wilkinson
Journal:  J Gen Microbiol       Date:  1965-06

8.  Some properties of nitrogen-fixing breis prepared from soybean root nodules.

Authors:  F J Bergersen
Journal:  Biochim Biophys Acta       Date:  1966-12-28

9.  The acetylene-ethylene assay for n(2) fixation: laboratory and field evaluation.

Authors:  R W Hardy; R D Holsten; E K Jackson; R C Burns
Journal:  Plant Physiol       Date:  1968-08       Impact factor: 8.340
  9 in total
  3 in total

1.  Effect of Ammonium Chloride and Methionine Sulfoximine on the Acetylene Reduction of Detached Root Nodules of Peas (Pisum sativum).

Authors:  F Houwaard
Journal:  Appl Environ Microbiol       Date:  1979-01       Impact factor: 4.792

2.  Whole-Cell Hybridization of Frankia Strains with Fluorescence- or Digoxigenin-Labeled, 16S rRNA-Targeted Oligonucleotide Probes.

Authors:  D Hahn; R I Amann; J Zeyer
Journal:  Appl Environ Microbiol       Date:  1993-06       Impact factor: 4.792

3.  Influence of ammonium chloride on the nitrogenase activity of nodulated pea plants (Pisum sativum).

Authors:  F Houwaard
Journal:  Appl Environ Microbiol       Date:  1978-06       Impact factor: 4.792
  3 in total

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