| Literature DB >> 8200847 |
Y Zhong1, K Enomoto, H Tobioka, Y Konishi, M Satoh, M Mori.
Abstract
A sequential decrease in the number of hepatocyte tight junctions during the course of rat hepatocarcinogenesis was demonstrated by immunohistochemistry with a new 7H6 monoclonal antibody generated in our laboratory. Semiquantitative analysis by confocal laser scanning microscopy revealed that the expression of 7H6 antigen was reduced in hyperplastic foci, hyperplastic nodules and hepatocellular carcinomas (HCC) to 43%, 28% and 25%, respectively, compared to corresponding normal liver tissues. 7H6 antigen was scarce in HCC with a trabecular pattern, whereas it was expressed intensely at the apical and basolateral membrane of HCC with a glandular pattern. Immunoblot analysis of 7H6 expression in hepatocellular carcinomas showed a decrease roughly coincident with that shown by immunohistochemistry. These results indicated, for the first time, that tight junctions decrease progressively during carcinogenesis, leading to disruption of cellular polarity and cellular adhesiveness.Entities:
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Year: 1994 PMID： 8200847 PMCID： PMC5919476 DOI： 10.1111/j.1349-7006.1994.tb02366.x
Source DB: PubMed Journal: Jpn J Cancer Res ISSN： 0910-5050