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Literature DB >> 8196630

scid cells efficiently integrate hairpin and linear DNA substrates.

Abstract

The scid mouse mutation affects V(D)J rearrangement and double-strand break repair. scid V(D)J rearrangement is characterized by defective coding joint formation which prevents the development of mature B and T cells. Hairpin DNA has been implicated in the formation of V(D)J coding joints. We found scid cells to be proficient in hairpin processing in the context of DNA integration. In addition, we found that the scid defect did not impair integration of linear DNA via nonhomologous recombination. Therefore, hairpin processing and integration of DNA into the genome are distinct from hypersensitivity to ionizing radiation and the defect in V(D)J recombination.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1994 PMID： 8196630 PMCID： PMC358754 DOI： 10.1128/mcb.14.6.3876-3883.1994

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

25 in total

1. Rearrangement of antigen receptor genes is defective in mice with severe combined immune deficiency.

Authors: W Schuler; I J Weiler; A Schuler; R A Phillips; N Rosenberg; T W Mak; J F Kearney; R P Perry; M J Bosma
Journal: Cell Date: 1986-09-26 Impact factor: 41.582

2. Linear DNA must have free ends to transform rat cells efficiently.

Authors: N Gusew; A Nepveu; P Chartrand
Journal: Mol Gen Genet Date: 1987-01

3. Modification of DNA ends can decrease end joining relative to homologous recombination in mammalian cells.

Authors: X B Chang; J H Wilson
Journal: Proc Natl Acad Sci U S A Date: 1987-07 Impact factor: 11.205

4. Pleiotropic effects of the scid mutation: effects on lymphoid differentiation and on repair of radiation damage.

Authors: R A Phillips; G M Fulop
Journal: Curr Top Microbiol Immunol Date: 1989 Impact factor: 4.291

5. The scid defect affects the final step of the immunoglobulin VDJ recombinase mechanism.

Authors: B A Malynn; T K Blackwell; G M Fulop; G A Rathbun; A J Furley; P Ferrier; L B Heinke; R A Phillips; G D Yancopoulos; F W Alt
Journal: Cell Date: 1988-08-12 Impact factor: 41.582

6. Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules.

Authors: K R Folger; E A Wong; G Wahl; M R Capecchi
Journal: Mol Cell Biol Date: 1982-11 Impact factor: 4.272

7. A severe combined immunodeficiency mutation in the mouse.

Authors: G C Bosma; R P Custer; M J Bosma
Journal: Nature Date: 1983-02-10 Impact factor: 49.962

8. The defect in murine severe combined immune deficiency: joining of signal sequences but not coding segments in V(D)J recombination.

Authors: M R Lieber; J E Hesse; S Lewis; G C Bosma; N Rosenberg; K Mizuuchi; M J Bosma; M Gellert
Journal: Cell Date: 1988-10-07 Impact factor: 41.582

9. The scid gene encodes a trans-acting factor that mediates the rejoining event of Ig gene rearrangement.

Authors: E A Hendrickson; D G Schatz; D T Weaver
Journal: Genes Dev Date: 1988-07 Impact factor: 11.361

10. Chicken IgL gene rearrangement involves deletion of a circular episome and addition of single nonrandom nucleotides to both coding segments.

Authors: W T McCormack; L W Tjoelker; L M Carlson; B Petryniak; C F Barth; E H Humphries; C B Thompson
Journal: Cell Date: 1989-03-10 Impact factor: 41.582

6 in total

scid cells efficiently integrate hairpin and linear DNA substrates.

1. Rearrangement of antigen receptor genes is defective in mice with severe combined immune deficiency.

2. Linear DNA must have free ends to transform rat cells efficiently.

3. Modification of DNA ends can decrease end joining relative to homologous recombination in mammalian cells.

4. Pleiotropic effects of the scid mutation: effects on lymphoid differentiation and on repair of radiation damage.

5. The scid defect affects the final step of the immunoglobulin VDJ recombinase mechanism.

6. Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules.

7. A severe combined immunodeficiency mutation in the mouse.

8. The defect in murine severe combined immune deficiency: joining of signal sequences but not coding segments in V(D)J recombination.

9. The scid gene encodes a trans-acting factor that mediates the rejoining event of Ig gene rearrangement.

10. Chicken IgL gene rearrangement involves deletion of a circular episome and addition of single nonrandom nucleotides to both coding segments.

1. Efficient nonhomologous and homologous recombination in scid cells.

2. DNA hairpin opening mediated by the RAG1 and RAG2 proteins.

3. Ku70, an essential gene, modulates the frequency of rAAV-mediated gene targeting in human somatic cells.

4. DNA-PK is essential only for coding joint formation in V(D)J recombination.

5. Mechanistic constraints on diversity in human V(D)J recombination.

6. scid cells are deficient in Ku and replication protein A phosphorylation by the DNA-dependent protein kinase.