Literature DB >> 8196481

An improved method to calculate adipose tissue interstitial substrate recovery for microdialysis studies.

P A Jansson1, T Veneman, N Nurjhan, J Gerich.   

Abstract

We simultaneously compared the conventional, time-consuming point of no net flux method for calculation of interstitial substrate recovery necessary for in vivo microdialysis studies with a simple isotopic method using rat epididymal fat pads. The recovery (%) calculated with the conventional method and the isotopic method for glucose (7.4 +/- 1.1 vs. 6.6 +/- 0.6), glycerol (23 +/- 4 vs. 26 +/- 5) and lactate (40 +/- 8 vs. 38 +/- 5), respectively, were not significantly different. Moreover, the overall correlation coefficient (N = 25) between the methods was 0.87, p < 0.001. We therefore conclude that the methods yield comparable results, and the more convenient isotopic method should become the method of choice for determining adipose tissue interstitial recovery for glucose, lactate and glycerol.

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Year:  1994        PMID： 8196481     DOI： 10.1016/0024-3205(94)90034-5

Source DB:  PubMed          Journal:  Life Sci        ISSN： 0024-3205            Impact factor:   5.037


  11 in total

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6.  Lifelong physical activity prevents an age-related reduction in arterial and skeletal muscle nitric oxide bioavailability in humans.

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