Literature DB >> 8195408

Enzyme-linked immunosorbent assay-enzyme-linked coagulation assay for detection of antibodies to Clostridium botulinum neurotoxins A, B, and E and solution-phase complexes.

G J Doellgast1, M X Triscott, G A Beard, J D Bottoms.   

Abstract

The solution-phase complex assay for toxins A, B, and E from Clostridium botulinum (Elcatech, Inc., Winston-Salem, N.C.) was modified to measure antibody. The addition of unlabeled polyclonal antibodies to a mixture consisting of toxin with chicken antibody and RVV-XA-labeled horse antibody reduces the sensitivity of detection of neurotoxin. This reduction in sensitivity can be used as a measure of the specific antibody titer.

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Year:  1994        PMID： 8195408      PMCID： PMC263141          DOI： 10.1128/jcm.32.3.851-853.1994

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  Evaluation of neutralizing antibodies to type A, B, E, and F botulinum toxins in sera from human recipients of botulinum pentavalent (ABCDE) toxoid.

Authors:  L S Siegel
Journal:  J Clin Microbiol       Date:  1989-08       Impact factor: 5.948

2.  Enzyme-linked immunosorbent assay and enzyme-linked coagulation assay for detection of Clostridium botulinum neurotoxins A, B, and E and solution-phase complexes with dual-label antibodies.

Authors:  G J Doellgast; G A Beard; J D Bottoms; T Cheng; B H Roh; M G Roman; P A Hall; M X Triscott
Journal:  J Clin Microbiol       Date:  1994-01       Impact factor: 5.948

3.  Sensitive enzyme-linked immunosorbent assay for detection of Clostridium botulinum neurotoxins A, B, and E using signal amplification via enzyme-linked coagulation assay.

Authors:  G J Doellgast; M X Triscott; G A Beard; J D Bottoms; T Cheng; B H Roh; M G Roman; P A Hall; J E Brown
Journal:  J Clin Microbiol       Date:  1993-09       Impact factor: 5.948

4.  Human immune response to botulinum pentavalent (ABCDE) toxoid determined by a neutralization test and by an enzyme-linked immunosorbent assay.

Authors:  L S Siegel
Journal:  J Clin Microbiol       Date:  1988-11       Impact factor: 5.948

5.  Antitoxin levels in botulism patients treated with trivalent equine botulism antitoxin to toxin types A, B, and E.

Authors:  C H Hatheway; J D Snyder; J E Seals; T A Edell; G E Lewis
Journal:  J Infect Dis       Date:  1984-09       Impact factor: 5.226
  5 in total
  3 in total

1.  Sensitive assay for measurement of antibodies to Clostridium botulinum neurotoxins A, B, and E: use of hapten-labeled-antibody elution to isolate specific complexes.

Authors:  G J Doellgast; J E Brown; J A Koufman; C L Hatheway
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

2.  Evaluation of lateral-flow Clostridium botulinum neurotoxin detection kits for food analysis.

Authors:  Shashi K Sharma; Brian S Eblen; Robert L Bull; Donald H Burr; Richard C Whiting
Journal:  Appl Environ Microbiol       Date:  2005-07       Impact factor: 4.792

3.  Detection of type A, B, E, and F Clostridium botulinum neurotoxins in foods by using an amplified enzyme-linked immunosorbent assay with digoxigenin-labeled antibodies.

Authors:  Shashi K Sharma; Joseph L Ferreira; Brian S Eblen; Richard C Whiting
Journal:  Appl Environ Microbiol       Date:  2006-02       Impact factor: 4.792
  3 in total

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