Literature DB >> 8195104

Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked.

R J Danaher1, E F Petricoin, D C Stein.   

Abstract

The Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene lsi-1 is preceded by a 281-bp non-protein-encoding sequence, lsi-3, that contains two pairs of inverted repeats. Gonococcal chromosomal lsi-xylE gene fusions were generated to measure the effect of the secondary structure on transcriptional attenuation. The data obtained indicate that lsi-3 and lsi-1 are not transcriptionally linked and therefore that lsi-3 is not involved in the regulation of lsi-1.

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Year:  1994        PMID： 8195104      PMCID： PMC205521          DOI： 10.1128/jb.176.11.3428-3432.1994

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  25 in total

1.  Plasmids with easily excisable xylE cassettes.

Authors:  D C Stein
Journal:  Gene       Date:  1992-08-01       Impact factor: 3.688

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

4.  The meta cleavage of catechol by Azotobacter species. 4-Oxalocrotonate pathway.

Authors:  J M Sala-Trepat; W C Evans
Journal:  Eur J Biochem       Date:  1971-06-11

5.  A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.

Authors:  C M Tsai; C E Frasch
Journal:  Anal Biochem       Date:  1982-01-01       Impact factor: 3.365

6.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

7.  Pyocin-resistant lipopolysaccharide mutans of Neisseria gonorrhoeae: alterations in sensitivity to normal human serum and polymyxin B.

Authors:  L F Guymon; M Esser; W M Shafer
Journal:  Infect Immun       Date:  1982-05       Impact factor: 3.441

8.  Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels.

Authors:  P J Hitchcock; T M Brown
Journal:  J Bacteriol       Date:  1983-04       Impact factor: 3.490

9.  Toxic activity of purified lipopolysaccharide of Neisseria gonorrhoeae for human fallopian tube mucosa.

Authors:  C R Gregg; M A Melly; C G Hellerqvist; J G Coniglio; Z A McGee
Journal:  J Infect Dis       Date:  1981-03       Impact factor: 5.226

10.  Immunological basis of serum resistance of Neisseria gonorrhoeae.

Authors:  H Schneider; J M Griffiss; G D Williams; G B Pier
Journal:  J Gen Microbiol       Date:  1982-01
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  3 in total

1.  Analysis of lipooligosaccharide biosynthesis in the Neisseriaceae.

Authors:  D Arking; Y Tong; D C Stein
Journal:  J Bacteriol       Date:  2001-02       Impact factor: 3.490

2.  Genetic basis of pyocin resistance in Neisseria gonorrhoeae.

Authors:  R C Sandlin; R J Danaher; D C Stein
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

3.  The lgtABCDE gene cluster, involved in lipooligosaccharide biosynthesis in Neisseria gonorrhoeae, contains multiple promoter sequences.

Authors:  Derek C Braun; Daniel C Stein
Journal:  J Bacteriol       Date:  2004-02       Impact factor: 3.490
  3 in total

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