Literature DB >> 8188629

An antibody for phosphorylated myosin light chain of smooth muscle: application to a biochemical study.

K Sakurada1, T Ikuhara, M Seto, Y Sasaki.   

Abstract

An antibody with specificity for the 20 kDa myosin light chain of smooth muscle phosphorylated by myosin light chain kinase was developed. The antibody was raised against the phosphorylated synthetic peptide, Lys-Lys-Arg-Pro-Gln-Arg-Ala-Thr-phospho-Ser-Asn-Val-Phe-Cys (residues 11-22 of the myosin light chain). This antibody only recognized the monophosphorylated myosin light chain at serine 19, i.e., with no detectable recognition of nonphosphorylated or diphosphorylated serine 19 or threonine 18. The specificity was tested by EIA assaying of myosin light chain kinase activity using a 96-well plate coated with the light chain. This EIA system was as sensitive as the radioisotopic method, and the newly developed non-radioisotopic method.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 8188629     DOI： 10.1093/oxfordjournals.jbchem.a124297

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  9 in total

1.  Rac1 links leading edge and uropod events through Rho and myosin activation during chemotaxis.

Authors:  Kersi N Pestonjamasp; Carol Forster; Chunxiang Sun; Elisabeth M Gardiner; Ben Bohl; Orion Weiner; Gary M Bokoch; Michael Glogauer
Journal:  Blood       Date:  2006-06-29       Impact factor: 22.113

2.  Myosin light chain diphosphorylation is enhanced by growth promotion of cultured smooth muscle cells.

Authors:  M Seto; K Sakurada; K E Kamm; J T Stull; Y Sasaki
Journal:  Pflugers Arch       Date:  1996-05       Impact factor: 3.657

3.  Inhibition of protein kinase C-mediated contraction by Rho kinase inhibitor fasudil in rabbit aorta.

Authors:  Erika Shimomura; Mitsuya Shiraishi; Takahiro Iwanaga; Minoru Seto; Yasuharu Sasaki; Masahiro Ikeda; Katsuaki Ito
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2004-10-01       Impact factor: 3.000

4.  The novel sphingosine 1-phosphate receptor AGR16 is coupled via pertussis toxin-sensitive and -insensitive G-proteins to multiple signalling pathways.

Authors:  K Gonda; H Okamoto; N Takuwa; Y Yatomi; H Okazaki; T Sakurai; S Kimura; R Sillard; K Harii; Y Takuwa
Journal:  Biochem J       Date:  1999-01-01       Impact factor: 3.857

5.  Essential role of rho kinase in the Ca2+ sensitization of prostaglandin F(2alpha)-induced contraction of rabbit aortae.

Authors:  Katsuaki Ito; Erika Shimomura; Takahiro Iwanaga; Mitsuya Shiraishi; Kazutoshi Shindo; Junji Nakamura; Hiromitsu Nagumo; Minoru Seto; Yasuharu Sasaki; Yoh Takuwa
Journal:  J Physiol       Date:  2003-02-01       Impact factor: 5.182

6.  Possible role of the protein kinase C/CPI-17 pathway in the augmented contraction of human myometrium after gestation.

Authors:  Hiroshi Ozaki; Katsuhiko Yasuda; Yoon-Sun Kim; Makoto Egawa; Hideharu Kanzaki; Hiroshi Nakazawa; Masatoshi Hori; Minoru Seto; Hideaki Karaki
Journal:  Br J Pharmacol       Date:  2003-10-27       Impact factor: 8.739

7.  Citron kinase, a Rho-dependent kinase, induces di-phosphorylation of regulatory light chain of myosin II.

Authors:  Shigeko Yamashiro; Go Totsukawa; Yoshihiko Yamakita; Yasuharu Sasaki; Pascal Madaule; Toshimaa Ishizaki; Shuh Narumiya; Fumio Matsumura
Journal:  Mol Biol Cell       Date:  2003-02-06       Impact factor: 4.138

8.  Specific localization of serine 19 phosphorylated myosin II during cell locomotion and mitosis of cultured cells.

Authors:  F Matsumura; S Ono; Y Yamakita; G Totsukawa; S Yamashiro
Journal:  J Cell Biol       Date:  1998-01-12       Impact factor: 10.539

9.  The NS5A/NS5 proteins of viruses from three genera of the family flaviviridae are phosphorylated by associated serine/threonine kinases.

Authors:  K E Reed; A E Gorbalenya; C M Rice
Journal:  J Virol       Date:  1998-07       Impact factor: 5.103
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.