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Literature DB >> 8187764

UV light-induced DNA synthesis arrest in HeLa cells is associated with changes in phosphorylation of human single-stranded DNA-binding protein.

M P Carty¹, M Zernik-Kobak, S McGrath, K Dixon.

Abstract

We show that DNA replication activity in extracts of human HeLa cells decreases following UV irradiation. Alterations in replication activity in vitro parallel the UV-induced block in cell cycle progression of these cells in culture. UV irradiation also induces specific changes in the pattern of phosphorylation of the 34 kDa subunit of a DNA replication protein, human single-stranded DNA-binding protein (hSSB). The appearance of a hyperphosphorylated form of hSSB correlates with reduced in vitro DNA replication activity in extracts of UV-irradiated cells. Replication activity can be restored to these extracts in vitro by addition of purified hSSB. These results suggest that UV-induced DNA synthesis arrest may be mediated in part through phosphorylation-related alterations in the activity of hSSB, an essential component of the DNA replication apparatus.

Entities: Chemical

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Year: 1994 PMID： 8187764 PMCID： PMC395063 DOI： 10.1002/j.1460-2075.1994.tb06487.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

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