Literature DB >> 8186421

Sex identification by polymerase chain reaction using a Y-autosome homologous primer set.

T Iida1, Y Nakahori, K Tanaka, O Tsutsumi, Y Taketani, Y Nakagome.   

Abstract

We have developed a one-step polymerase chain reaction (PCR) technique which detects a sequence on the human Y chromosome and an autosomal sequence in one reaction. The method is very reliable for the sex determination, as the detection of the autosome-specific signal ensures the presence of DNA in the specimen even in the absence of the Y-specific signal.

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Year:  1993        PMID: 8186421     DOI: 10.1007/BF01907990

Source DB:  PubMed          Journal:  Jpn J Hum Genet        ISSN: 0916-8478


  6 in total

1.  PCR detection of structurally abnormal Y chromosomes.

Authors:  S Nagafuchi; S Seki; Y Nakahori; T Tamura; H Numabe; Y Nakagome
Journal:  Jpn J Hum Genet       Date:  1992-09

2.  Sex identification by polymerase chain reaction using X-Y homologous primer.

Authors:  Y Nakahori; K Hamano; M Iwaya; Y Nakagome
Journal:  Am J Med Genet       Date:  1991-06-15

3.  A rapid method for detection of Y-chromosomal DNA from dried blood specimens by the polymerase chain reaction.

Authors:  M Witt; R P Erickson
Journal:  Hum Genet       Date:  1989-06       Impact factor: 4.132

4.  An improved method for prenatal diagnosis of genetic diseases by analysis of amplified DNA sequences. Application to hemophilia A.

Authors:  S C Kogan; M Doherty; J Gitschier
Journal:  N Engl J Med       Date:  1987-10-15       Impact factor: 91.245

5.  Sex identification in fresh blood and dried bloodstains by a nonisotopic deoxyribonucleic acid (DNA) analyzing technique.

Authors:  R Kobayashi; H Nakauchi; Y Nakahori; Y Nakagome; S Matsuzawa
Journal:  J Forensic Sci       Date:  1988-05       Impact factor: 1.832

6.  Cosmid clones derived from both euchromatic and heterochromatic regions of the human Y chromosome.

Authors:  J Wolfe; R P Erickson; P W Rigby; P N Goodfellow
Journal:  EMBO J       Date:  1984-09       Impact factor: 11.598

  6 in total

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