Literature DB >> 8181838

Detection of Clostridium difficile toxin by enzyme immunoassay, tissue culture test and culture.

O Liesenfeld1, F Saeger, H Hahn.   

Abstract

Clostridium difficile toxin is frequently encountered in patients with antibiotic-associated diarrhea. A commercially available enzyme immunoassay (EIA) detecting toxins A and B was evaluated, screening 148 stool specimens specifically submitted for the detection of C. difficile. A sensitivity of 100% and a specificity of 97.5% were found compared to a tissue culture assay. The overall prevalence of C. difficile toxin was 22.1%. Cephalosporins had been administered significantly more often to toxin-positive patients. The EIA proved to be a suitable test for the detection of C. difficile toxin, revealing high prevalences in specifically submitted clinical specimens.

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Year:  1994        PMID： 8181838     DOI： 10.1007/bf01780761

Source DB:  PubMed          Journal:  Infection        ISSN： 0300-8126            Impact factor:   3.553


  18 in total

1.  Cytotoxicity assay in antibiotic-associated colitis.

Authors:  T W Chang; M Lauermann; J G Bartlett
Journal:  J Infect Dis       Date:  1979-11       Impact factor: 5.226

2.  Characterization of toxins A and B of Clostridium difficile with monoclonal antibodies.

Authors:  D M Lyerly; C J Phelps; J Toth; T D Wilkins
Journal:  Infect Immun       Date:  1986-10       Impact factor: 3.441

3.  Multicenter evaluation of a new enzyme immunoassay for detection of Clostridium difficile enterotoxin A.

Authors:  P C De Girolami; P A Hanff; K Eichelberger; L Longhi; H Teresa; J Pratt; A Cheng; J M Letourneau; G M Thorne
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948

4.  Three-year prevalence of enteropathogenic bacteria in an urban patient population in Germany.

Authors:  O Liesenfeld; T Weinke; H Hahn
Journal:  Infection       Date:  1993 Mar-Apr       Impact factor: 3.553

5.  Antimicrobial agents and Clostridium difficile in acute enteric disease: epidemiological data from Sweden, 1980-1982.

Authors:  B Aronsson; R Möllby; C E Nord
Journal:  J Infect Dis       Date:  1985-03       Impact factor: 5.226

6.  Commercial latex agglutination test for detection of Clostridium difficile-associated diarrhea.

Authors:  M T Kelly; S G Champagne; C H Sherlock; M A Noble; H J Freeman; J A Smith
Journal:  J Clin Microbiol       Date:  1987-07       Impact factor: 5.948

7.  Inappropriate testing for diarrheal diseases in the hospital.

Authors:  D L Siegel; P H Edelstein; I Nachamkin
Journal:  JAMA       Date:  1990-02-16       Impact factor: 56.272

8.  Development of a rapid enzyme immunoassay for Clostridium difficile toxin A and its use in the diagnosis of C. difficile-associated disease.

Authors:  J R DiPersio; F J Varga; D L Conwell; J A Kraft; K J Kozak; D H Willis
Journal:  J Clin Microbiol       Date:  1991-12       Impact factor: 5.948

9.  Application of rejection criteria for stool cultures for bacterial enteric pathogens.

Authors:  K Fan; A J Morris; L B Reller
Journal:  J Clin Microbiol       Date:  1993-08       Impact factor: 5.948

10.  Effects of Clostridium difficile toxins given intragastrically to animals.

Authors:  D M Lyerly; K E Saum; D K MacDonald; T D Wilkins
Journal:  Infect Immun       Date:  1985-02       Impact factor: 3.441
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  1 in total

1.  Culture of intestinal biopsy specimens and stool culture for detection of bacterial enteropathogens in patients infected with human immunodeficiency virus. The Berlin Diarrhea/Wasting Syndrome Study Group.

Authors:  O Liesenfeld; T Schneider; W Schmidt; J Sandforth; T Weinke; M Zeitz; E O Riecken; R Ullrich
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948
  1 in total

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