Literature DB >> 8180734

Screening techniques for detecting allelic variation in DNA sequences.

E P Lessa1, G Applebaum.   

Abstract

This article reviews four 'DNA screening techniques', namely heteroduplex analysis, single-strand conformational polymorphism (SSCP), denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) as tools for the study of allelic variation in natural populations. The resolving power, advantages, and limitations of each technique are discussed and compared. We also provide some criteria for choosing among techniques and illustrate some practical issues with examples taken primarily from our own laboratory experience.

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Year:  1993        PMID： 8180734     DOI： 10.1111/j.1365-294x.1993.tb00006.x

Source DB:  PubMed          Journal:  Mol Ecol        ISSN： 0962-1083            Impact factor:   6.185


  16 in total

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