Literature DB >> 817846

[A genetic transfer system in a cyanophyte of the genus Aphanocapsa].

C Astier, F Espardellier.   

Abstract

The possibility of a genetic transfer, through a process of transformation, has been shown in a unicellular, facultative chemoheterotroph blue-green Algae, Aphanocapsa 6714. Similarly to the process described with Anacystis nidulans, the recipient strain becomes competent when the growth reaches its second, slower, exponential phase. The genetic marker conferring resistance to p-F-phenylalanine has been transferred with a frequency 20 to 100 times higher than that of the spontaneous mutations. The efficiency of transformation decreases by a factor of 70% when the transforming extract, which contains both DNA and RNA, is pretreated with DNase. This transfer process is compared to that observed with A. nidulans by several authors.

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Year:  1976        PMID: 817846

Source DB:  PubMed          Journal:  C R Acad Hebd Seances Acad Sci D


  7 in total

Review 1.  Genetics of Rhodospirillaceae.

Authors:  V A Saunders
Journal:  Microbiol Rev       Date:  1978-06

2.  Identification of facultatively heterotrophic, N2-fixing cyanobacteria able to receive plasmid vectors from Escherichia coli by conjugation.

Authors:  E Flores; C P Wolk
Journal:  J Bacteriol       Date:  1985-06       Impact factor: 3.490

3.  Transformation in the cyanobacterium Synechococcus R2: improvement of efficiency; role of the pUH24 plasmid.

Authors:  F Chauvat; C Astier; F Vedel; F Joset-Espardellier
Journal:  Mol Gen Genet       Date:  1983

4.  Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria.

Authors:  C P Wolk; A Vonshak; P Kehoe; J Elhai
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

5.  Introduction of transposon Tn901 into a plasmid of Anacystis nidulans: preparation for cloning in cyanobacteria.

Authors:  C A van den Hondel; S Verbeek; A van der Ende; P J Weisbeek; W E Borrias; G A van Arkel
Journal:  Proc Natl Acad Sci U S A       Date:  1980-03       Impact factor: 11.205

6.  Ethionine-resistant mutants of the filamentous blue-green alga Plectonema boryanum.

Authors:  W M Hentschel; J L Farmer; W R Andersen
Journal:  J Bacteriol       Date:  1978-03       Impact factor: 3.490

7.  Vectors for cloning in cyanobacteria: construction and characterization of two recombinant plasmids capable of transformation of Escherichia coli K12 and Anacystis nidulans R2.

Authors:  C J Kuhlemeier; W E Borrias; C A van den Hondel; G A van Arkel
Journal:  Mol Gen Genet       Date:  1981
  7 in total

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