Literature DB >> 8176751

Crystallization and preliminary crystallographic studies of Gi alpha 1 and mutants of Gi alpha 1 in the GTP and GDP-bound states.

D E Coleman1, E Lee, M B Mixon, M E Linder, A M Berghuis, A G Gilman, S R Sprang.   

Abstract

Several different crystal forms of Gi alpha 1 have been grown and analyzed. Crystals of native protein containing bound GTP gamma S belong to space group P3(1)2(1) or P3(2)2(1) with cell dimensions a,b = 80.6 A and c = 106.3 A and diffract to a resolution of 1.9 A using synchrotron radiation. Crystals of native protein containing bound GDP belong to space group I4 with cell dimensions a,b = 121.3 A, and c = 67.7 A and diffract to 3.0 A. Data sets from crystals grown using mutant proteins have also been obtained and characterized.

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Year:  1994        PMID： 8176751     DOI： 10.1006/jmbi.1994.1320

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  14 in total

1.  Crystallization and preliminary X-ray crystallographic analysis of the receptor-uncoupled mutant of Galphai1.

Authors:  Tomohito Morikawa; Ayumu Muroya; Yoshitaka Nakajima; Takeshi Tanaka; Keiko Hirai; Shigetoshi Sugio; Kaori Wakamatsu; Toshiyuki Kohno
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-01-27

2.  Analysis of the mechanism of the Serratia nuclease using site-directed mutagenesis.

Authors:  P Friedhoff; B Kolmes; O Gimadutdinow; W Wende; K L Krause; A Pingoud
Journal:  Nucleic Acids Res       Date:  1996-07-15       Impact factor: 16.971

3.  Activated alleles of the Schizosaccharomyces pombe gpa2+ Galpha gene identify residues involved in GDP-GTP exchange.

Authors:  F Douglas Ivey; Francis X Taglia; Fan Yang; Matthew M Lander; David A Kelly; Charles S Hoffman
Journal:  Eukaryot Cell       Date:  2010-02-05

4.  Mutation of cysteine 214 in Gi1 alpha subunit abolishes its endogenous GTPase activity.

Authors:  Yuren Wang; Gregory Tawa; Deborah Smith; Girija Krishnamurthy; Kathleen H Young
Journal:  Biochem J       Date:  2004-05-01       Impact factor: 3.857

5.  Effects of mutations in the N terminal region of the yeast G protein alpha-subunit Gpa1p on signaling by pheromone receptors.

Authors:  M Roginskaya; S M Connelly; K S Kim; D Patel; M E Dumont
Journal:  Mol Genet Genomics       Date:  2004-02-07       Impact factor: 3.291

6.  Mechanism of GTP hydrolysis by G-protein alpha subunits.

Authors:  C Kleuss; A S Raw; E Lee; S R Sprang; A G Gilman
Journal:  Proc Natl Acad Sci U S A       Date:  1994-10-11       Impact factor: 11.205

7.  GIV/girdin links vascular endothelial growth factor signaling to Akt survival signaling in podocytes independent of nephrin.

Authors:  Honghui Wang; Taro Misaki; Vanessa Taupin; Akiko Eguchi; Pradipta Ghosh; Marilyn G Farquhar
Journal:  J Am Soc Nephrol       Date:  2014-07-10       Impact factor: 10.121

8.  GoLoco motif proteins binding to Galpha(i1): insights from molecular simulations.

Authors:  Kamil Khafizov
Journal:  J Mol Model       Date:  2009-05-14       Impact factor: 1.810

9.  Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding.

Authors:  David Goricanec; Ralf Stehle; Pascal Egloff; Simina Grigoriu; Andreas Plückthun; Gerhard Wagner; Franz Hagn
Journal:  Proc Natl Acad Sci U S A       Date:  2016-06-13       Impact factor: 11.205

10.  The nucleotide exchange factor Ric-8A is a chaperone for the conformationally dynamic nucleotide-free state of Gαi1.

Authors:  Celestine J Thomas; Klára Briknarová; Jonathan K Hilmer; Navid Movahed; Brian Bothner; John P Sumida; Gregory G Tall; Stephen R Sprang
Journal:  PLoS One       Date:  2011-08-11       Impact factor: 3.240
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