| Literature DB >> 8174710 |
K M Kim1, D K Kim, Y M Park, C K Kim, D S Na.
Abstract
Annexin-I is a calcium dependent phospholipid binding and phospholipase A2 (PLA2) inhibitory protein. A 'substrate depletion' model has been proposed for the mechanism of PLA2 inhibition by annexin-I in studies with 14 to 18 kDa PLA2s. Herein, we have studied the inhibition mechanism using 100 kDa cytosolic PLA2 from porcine spleen. The inhibition has been measured at various substrate and calcium ion concentrations. The pattern of PLA2 inhibition by annexin-I was consistent with a 'specific interaction' mechanism rather than the 'substrate depletion' model. Apparent contraction with previous studies can be explained by the calcium-dependent binding of annexin-I to the substrate.Entities:
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Year: 1994 PMID： 8174710 DOI： 10.1016/0014-5793(94)80566-0
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124