| Literature DB >> 8171321 |
J H Postlethwait1, S L Johnson, C N Midson, W S Talbot, M Gates, E W Ballinger, D Africa, R Andrews, T Carl, J S Eisen.
Abstract
To facilitate molecular genetic analysis of vertebrate development, haploid genetics was used to construct a recombination map for the zebrafish Danio (Brachydanio) rerio. The map consists of 401 random amplified polymorphic DNAs (RAPDs) and 13 simple sequence repeats spaced at an average interval of 5.8 centimorgans. Strategies that exploit the advantages of haploid genetics and RAPD markers were developed that quickly mapped lethal and visible mutations and that placed cloned genes on the map. This map is useful for the position-based cloning of mutant genes, the characterization of chromosome rearrangements, and the investigation of evolution in vertebrate genomes.Entities:
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Year: 1994 PMID： 8171321 DOI： 10.1126/science.8171321
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728