| Literature DB >> 8169951 |
Abstract
Mixtures of ethanol, 2-propanol and 2-butanol can be used as a cryoglue to mount vitrified biological specimens for ultrathin cryosectioning. Brought directly from room temperature to a cutting support at 140 K in the cold chamber of a cryoultramicrotome, these alcohols stiffen to a viscous and gluey consistency allowing the attachment or embedding of a vitreous biological sample. The mass hardens at lower temperatures fixing the sample well for microtomy. With ethanol: 2-propanol (2:3), samples are applied at 140 K and ultrathin cutting can be done at 115 K.Entities:
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Year: 1994 PMID： 8169951 DOI： 10.1111/j.1365-2818.1994.tb03436.x
Source DB: PubMed Journal: J Microsc ISSN： 0022-2720 Impact factor: 1.758