Literature DB >> 8168626

Stereochemical course of the hydrolysis reaction catalyzed by chitinases A1 and D from Bacillus circulans WL-12.

S Armand1, H Tomita, A Heyraud, C Gey, T Watanabe, B Henrissat.   

Abstract

Chitinases A1 and D were purified from the periplasmic proteins produced by Escherichia coli HB101 harbouring recombinant plasmids carrying respectively the chiA and chiD genes of Bacillus circulans WL-12. HPLC analysis indicated that during the hydrolysis of chitotriose, both chitinases initially produce N-acetylglucosamine and only one anomer of chitobiose. 1H NMR spectroscopy of the hydrolysis of chitotetraitol showed that this anomer corresponds to beta-chitobiose, demonstrating that chitinases A1 and D act by a molecular mechanism that retains the anomeric configuration. This mechanism is similar to that of lysozymes although both chitinases belong to a family of proteins sharing no demonstrable amino acid sequence similarity with lysozymes.

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Year:  1994        PMID： 8168626     DOI： 10.1016/0014-5793(94)80314-5

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  14 in total

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Authors:  D M van Aalten; D Komander; B Synstad; S Gåseidnes; M G Peter; V G Eijsink
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-31       Impact factor: 11.205

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4.  Expression, purification, crystallization and preliminary crystallographic analysis of a GH20 β-N-acetylglucosaminidase from the marine bacterium Vibrio harveyi.

Authors:  Piyanat Meekrathok; Marco Bürger; Arthur T Porfetye; Ingrid R Vetter; Wipa Suginta
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5.  Genetic analysis of the chitinase system of Serratia marcescens 2170.

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7.  Structure of chitinase D from Serratia proteamaculans reveals the structural basis of its dual action of hydrolysis and transglycosylation.

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8.  Substrate binding modes and anomer selectivity of chitinase A from Vibrio harveyi.

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Journal:  J Chem Biol       Date:  2009-05-28

9.  Mode of action and antifungal properties of two cold-adapted chitinases.

Authors:  Konstantinos Mavromatis; Matteo Lorito; Sheridan L Woo; Vassilis Bouriotis
Journal:  Extremophiles       Date:  2003-07-15       Impact factor: 2.395

10.  Mutation of Trp137 to glutamate completely removes transglycosyl activity associated with the Aspergillus fumigatus AfChiB1.

Authors:  Yang Lü; Haitao Yang; Hongyan Hu; Ying Wang; Zihe Rao; Cheng Jin
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