Literature DB >> 8167853

Segregation analysis of random amplified polymorphic DNA (RAPD) markers in Picea abies Karst.

G Bucci1, P Menozzi.   

Abstract

The reliability of arbitrarily primed amplification products was tested. The segregation analysis of 266 amplification products obtained using 17 different 10-mer oligonucleotides in 34 megagametophytes from a single tree of Picea abies was carried out. Fifty-four out of the 165 variable bands fit the 1:1 segregation ratio expected for Mendelian traits. The segregation ratio of a subset of six RAPD markers in five other individuals from the same population confirmed their genetic nature. Our results strengthen the evidence previously reported that RAPDs markers can be considered Mendelian traits useful in the detection of genetic variability among both different individuals and populations.

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Year:  1993        PMID: 8167853     DOI: 10.1111/j.1365-294x.1993.tb00012.x

Source DB:  PubMed          Journal:  Mol Ecol        ISSN: 0962-1083            Impact factor:   6.185


  3 in total

1.  Comparative study of interspecific genetic divergence and phylogenic analysis of genus Jatropha by RAPD and AFLP: genetic divergence and phylogenic analysis of genus Jatropha.

Authors:  D V N Sudheer Pamidiamarri; Nirali Pandya; Muppala P Reddy; T Radhakrishnan
Journal:  Mol Biol Rep       Date:  2008-04-30       Impact factor: 2.316

2.  Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas-fir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica).

Authors:  Y Tsumura; K Ohba; S H Strauss
Journal:  Theor Appl Genet       Date:  1996-01       Impact factor: 5.699

3.  A genetic linkage map of Picea abies Karst., based on RAPD markers, as a tool in population genetics.

Authors:  G Binelli; G Bucci
Journal:  Theor Appl Genet       Date:  1994-06       Impact factor: 5.699

  3 in total

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