Literature DB >> 816745

Human fallopian tubes in organ culture: preparation, maintenance, and quantitation of damage by pathogenic microorganisms.

Z A McGee, A P Johnson, D Taylor-Robinson.   

Abstract

An experimental organ culture model has been developed in which rates of damage to fallopian tube mucosa by agents of sexually transmitted diseases can be quantitated. This required assessment of the effect on organ cultures of such variables as the anatomic area of the fallopian tube employed, endocrinological status of the donor, and composition of media. Organ cultures established from the ampulla of tubes of nonpregnant, premenopausal women and maintained with Eagle minimal essential medium containing 0.05 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid buffer were most suitable for quantitative studies. Various means of quantitating damage to fallopian tube mucosa were evaluated. Of these, sequential estimations of ciliary vigor and percentage of the periphery of fallopian tube pieces with active cilia proved the most successful. To assess the specificity of the model, fallopian tube organ cultures were infected with Neisseria gonorrhoeae or N. pharyngis, a commensal Neisseria. Damage by N. gonorrhoeae was significantly greater than that by N. pharyngis at each period of observation (P less than 0.001), suggesting that, by using the model, differences in virulence can be determined in vitro that reflect differences in virulence for humans. This model is a potentially valuable tool for studying the interaction of human genital mucosa with known or suspected agents of sexually transmitted diseases.

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Year:  1976        PMID: 816745      PMCID: PMC420652          DOI: 10.1128/iai.13.2.608-618.1976

Source DB:  PubMed          Journal:  Infect Immun        ISSN: 0019-9567            Impact factor:   3.441


  29 in total

1.  Effect of a toxin produced by Haemophilus influenzae on ciliated respiratory epithelium.

Authors:  F W Denny
Journal:  J Infect Dis       Date:  1974-02       Impact factor: 5.226

2.  A method for standardized studies of mucociliary activity.

Authors:  U Mercke; C H Håkansson; N G Toremalm
Journal:  Acta Otolaryngol       Date:  1974 Jul-Aug       Impact factor: 1.494

3.  Antigenically distinct populations of Neisseria gonorrhoeae: isolation and characterization of the responsible determinants.

Authors:  M A Apicella
Journal:  J Infect Dis       Date:  1974-12       Impact factor: 5.226

4.  Neisseria gonorrhoeae: effects of systemic immunization on resistance of chimpanzees to urethral infection.

Authors:  R J Arko; S J Kraus; W J Brown; T M Buchanan; U S Kuhn
Journal:  J Infect Dis       Date:  1974-08       Impact factor: 5.226

5.  Low antibiotic resistance of gonococci causing disseminated infection.

Authors:  P J Wiesner; H H Handsfield; K K Holmes
Journal:  N Engl J Med       Date:  1973-06-07       Impact factor: 91.245

6.  The human fallopian tube: a laboratory model for gonococcal infection.

Authors:  M E Ward; P J Watt; J N Robertson
Journal:  J Infect Dis       Date:  1974-06       Impact factor: 5.226

7.  Search for thymidine phosphorylase, nucleoside deoxyribosyltransferase and thymidine kinase in genus Neisseria.

Authors:  S Jyssum
Journal:  Acta Pathol Microbiol Scand B Microbiol Immunol       Date:  1974-02

8.  Isolation and immunological characterization of a unique toxic cytoplasmic polymer of type 1 Neisseria gonorrhoeae.

Authors:  A Cheng; J B Kwapinski; A R Ronald
Journal:  Can J Microbiol       Date:  1974-08       Impact factor: 2.419

9.  Activities of some enzymes concerning pyruvate metabolism in Neisseria.

Authors:  E Holten; K Jyssum
Journal:  Acta Pathol Microbiol Scand B Microbiol Immunol       Date:  1974-12

10.  Antigenic heterogeneity of gonococcal pili.

Authors:  T M Buchanan
Journal:  J Exp Med       Date:  1975-06-01       Impact factor: 14.307

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  33 in total

1.  Role of M protein in adherence of group A streptococci.

Authors:  M G Caparon; D S Stephens; A Olsén; J R Scott
Journal:  Infect Immun       Date:  1991-05       Impact factor: 3.441

2.  Differential intracellular efficacies of ciprofloxacin and cefixime against Neisseria gonorrhoeae in human fallopian tube organ culture.

Authors:  J P Phanucharas; G L Gorby
Journal:  Antimicrob Agents Chemother       Date:  1997-07       Impact factor: 5.191

3.  Model for invasion of human tissue culture cells by Neisseria gonorrhoeae.

Authors:  J H Shaw; S Falkow
Journal:  Infect Immun       Date:  1988-06       Impact factor: 3.441

Review 4.  The molecular mechanisms used by Neisseria gonorrhoeae to initiate infection differ between men and women.

Authors:  Jennifer L Edwards; Michael A Apicella
Journal:  Clin Microbiol Rev       Date:  2004-10       Impact factor: 26.132

5.  Analysis of damage to human ciliated nasopharyngeal epithelium by Neisseria meningitidis.

Authors:  D S Stephens; A M Whitney; M A Melly; L H Hoffman; M M Farley; C E Frasch
Journal:  Infect Immun       Date:  1986-02       Impact factor: 3.441

6.  Attachment of pathogenic Neisseria to human mucosal surfaces: role in pathogenesis.

Authors:  D S Stephens; Z A McGee; M A Melly; L H Hoffman; C R Gregg
Journal:  Infection       Date:  1982       Impact factor: 3.553

7.  Quantification of bacterial internalization by host cells using a beta-lactamase reporter strain: Neisseria gonorrhoeae invasion into cervical epithelial cells requires bacterial viability.

Authors:  Samuel E Bish; Wenxia Song; Daniel C Stein
Journal:  Microbes Infect       Date:  2008-07-17       Impact factor: 2.700

8.  Identification and preliminary characterization of a cytotoxin isolated from Mobiluncus spp.

Authors:  A W Taylor-Robinson; S P Borriello; D Taylor-Robinson
Journal:  Int J Exp Pathol       Date:  1993-08       Impact factor: 1.925

9.  Localization of gonococcal lipopolysaccharide and its relationship to toxic damage in human fallopian tube mucosa.

Authors:  M D Cooper; P A McGraw; M A Melly
Journal:  Infect Immun       Date:  1986-02       Impact factor: 3.441

10.  Cytopathic effects of the pathogenic Neisseria. Studies using human fallopian tube organ cultures and human nasopharyngeal organ cultures.

Authors:  D S Stephens; Z A McGee; M D Cooper
Journal:  Antonie Van Leeuwenhoek       Date:  1987       Impact factor: 2.271

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