AIM: To develop a method to detect HIV-1 viral RNA by amplifying a specific nucleic acid sequence. METHOD: The nucleic acid sequence-based amplification (NASBA) method uses the simultaneous activity of avian myeloblastosis virus reverse transcriptase, T7 RNA polymerase and RNase H to amplify a specific nucleic acid target sequence. VALIDATION: An in vitro cultured HIV-1 stock solution was used to validate the NASBA method and determine the variation in RNA measurement. CONCLUSION: Although NASBA is theoretically capable of specific amplification of RNA or DNA, it is most suitable for amplification of RNA, and therefore for detection of HIV-1 viral RNA.
AIM: To develop a method to detect HIV-1 viral RNA by amplifying a specific nucleic acid sequence. METHOD: The nucleic acid sequence-based amplification (NASBA) method uses the simultaneous activity of avian myeloblastosis virus reverse transcriptase, T7 RNA polymerase and RNase H to amplify a specific nucleic acid target sequence. VALIDATION: An in vitro cultured HIV-1 stock solution was used to validate the NASBA method and determine the variation in RNA measurement. CONCLUSION: Although NASBA is theoretically capable of specific amplification of RNA or DNA, it is most suitable for amplification of RNA, and therefore for detection of HIV-1 viral RNA.
Authors: Jos J A M Weusten; Wim M Carpay; Tom A M Oosterlaken; Martien C A van Zuijlen; Paul A van de Wiel Journal: Nucleic Acids Res Date: 2002-03-15 Impact factor: 16.971
Authors: Jos J A M Weusten; Pieter A W M Wouters; Martien C A van Zuijlen; Paul A van de Wiel Journal: Nucleic Acids Res Date: 2002-12-15 Impact factor: 16.971
Authors: H J de Haard; B Kazemier; M J Koolen; L J Nijholt; R H Meloen; B van Gemen; H R Hoogenboom; J W Arends Journal: Clin Diagn Lab Immunol Date: 1998-09
Authors: S A Morré; P T Sillekens; M V Jacobs; S de Blok; J M Ossewaarde; P van Aarle; B van Gemen; J M Walboomers; C J Meijer; A J van den Brule Journal: Mol Pathol Date: 1998-06