Physiological evidence for ipsilateral inhibition in the lateral superior olive: synaptic responses in mouse brain slice.

The incidence of ipsilateral inhibition in the lateral superior olive (LSO) was examined in a brain slice preparation of the mouse superior olivary complex. A 400 microns brain slice was taken in the frontal plane and maintained in a warm, oxygenated saline solution. Intracellular recordings were made from the LSO with micropipettes filled with 4 M potassium acetate. Synaptic responses were elicited by electrical stimulation of the trapezoid body in different slices at various locations between the cochlear nucleus and the ipsilateral superior olivary complex (SOC). The results show that ipsilateral stimulation can evoke inhibitory as well as excitatory postsynaptic potentials. The ipsilateral IPSPs have short latencies and are elicited by stimulation of the trapezoid body at any point along its course between cochlear nucleus and LSO. Short-latency IPSPs can also be produced by direct stimulation of the ventral cochlear nucleus itself. Ipsilateral IPSPs are blocked by low concentrations of the glycine antagonist, strychnine. In addition, bath application of sodium pentobarbital in one case eliminated ipsilateral IPSPs without eliminating EPSPs. The results suggest that there is a rapidly conducting, glycinergic pathway from cochlear nucleus through the trapezoid body to the LSO on the same side of the brain. This pathway is probably served by either a direct projection from the ventral cochlear nucleus to the LSO or an indirect one from cochlear nucleus to LSO through the lateral nucleus of the trapezoid body (LNTB).