Literature DB >> 8156164

MULTIPRINS: a method for multicolour primed in situ labelling.

E V Volpi1, A Baldini.   

Abstract

With this fast and simple MULTIPRINS protocol we show simultaneous mapping of multiple repetitive sequences by sequential cycles of primed in situ (PRINS) labelling with various primers and reporter molecules. Differentially labelled DNA sequences synthesized in situ were visualized by directly incorporated rhodamine-4-dUTP, by fluorescein isothiocyanate and Cy5 using digital imaging microscopy. This further development of the PRINS method enhances its potential as an alternative to traditional in situ hybridization.

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Year:  1993        PMID： 8156164     DOI： 10.1007/bf00710131

Source DB:  PubMed          Journal:  Chromosome Res        ISSN： 0967-3849            Impact factor:   5.239


  9 in total

1.  Chromosomal assignment of human YAC clones by fluorescence in situ hybridization: use of single-yeast-colony PCR and multiple labeling.

Authors:  A Baldini; M Ross; D Nizetic; R Vatcheva; E A Lindsay; H Lehrach; M Siniscalco
Journal:  Genomics       Date:  1992-09       Impact factor: 5.736

2.  In situ hybridization banding of human chromosomes with Alu-PCR products: a simultaneous karyotype for gene mapping studies.

Authors:  A Baldini; D C Ward
Journal:  Genomics       Date:  1991-04       Impact factor: 5.736

3.  Fast one-step procedure for the detection of nucleic acids in situ by primer-induced sequence-specific labeling with fluorescein-12-dUTP.

Authors:  J Koch; J Mogensen; S Pedersen; H Fischer; J Hindkjaer; S Kølvraa; L Bolund
Journal:  Cytogenet Cell Genet       Date:  1992

4.  An improved method for chromosome-specific labeling of alpha satellite DNA in situ by using denatured double-stranded DNA probes as primers in a primed in situ labeling (PRINS) procedure.

Authors:  J Koch; J Hindkjaer; J Mogensen; S Kølvraa; L Bolund
Journal:  Genet Anal Tech Appl       Date:  1991-09

5.  A human alpha satellite DNA subset specific for chromosome 12.

Authors:  A Baldini; M Rocchi; N Archidiacono; O J Miller; D A Miller
Journal:  Am J Hum Genet       Date:  1990-04       Impact factor: 11.025

6.  A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes.

Authors:  R K Moyzis; J M Buckingham; L S Cram; M Dani; L L Deaven; M D Jones; J Meyne; R L Ratliff; J R Wu
Journal:  Proc Natl Acad Sci U S A       Date:  1988-09       Impact factor: 11.205

7.  A human centromere antigen (CENP-B) interacts with a short specific sequence in alphoid DNA, a human centromeric satellite.

Authors:  H Masumoto; H Masukata; Y Muro; N Nozaki; T Okazaki
Journal:  J Cell Biol       Date:  1989-11       Impact factor: 10.539

8.  Highly conserved repetitive DNA sequences are present at human centromeres.

Authors:  D L Grady; R L Ratliff; D L Robinson; E C McCanlies; J Meyne; R K Moyzis
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-01       Impact factor: 11.205

9.  Oligonucleotide-primed in situ DNA synthesis (PRINS): a method for chromosome mapping, banding, and investigation of sequence organization.

Authors:  J Gosden; D Hanratty; J Starling; J Fantes; A Mitchell; D Porteous
Journal:  Cytogenet Cell Genet       Date:  1991
  9 in total
  2 in total

1.  Ultra-rapid multicolor PRINS protocol for chromosome detection in human sperm.

Authors:  F Pellestor; S Malki; B Andréo; G Lefort
Journal:  Chromosome Res       Date:  2002       Impact factor: 5.239

2.  Comparison of FISH PRINS, and conventional and fluorescent PCR for single-cell sexing: suitability for preimplantation genetic diagnosis.

Authors:  I Findlay; N Corby; A Rutherford; P Quirke
Journal:  J Assist Reprod Genet       Date:  1998-05       Impact factor: 3.412
  2 in total

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