| Literature DB >> 8144479 |
Abstract
An amber suppressor variant of Bacillus subtilis tyrosyl-tRNA was constructed and placed under control of the isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible Pspac promoter. Addition of IPTG resulted in a 50-fold increase in the expression of an rpsD-lacZ fusion containing a UAG amber codon. This system permitted isolation of a conditional lethal mutant which required IPTG for growth.Entities:
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Year: 1994 PMID: 8144479 PMCID: PMC205319 DOI: 10.1128/jb.176.7.2108-2110.1994
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490