Literature DB >> 8144479

Inducible amber suppressor for Bacillus subtilis.

F J Grundy1, T M Henkin.   

Abstract

An amber suppressor variant of Bacillus subtilis tyrosyl-tRNA was constructed and placed under control of the isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible Pspac promoter. Addition of IPTG resulted in a 50-fold increase in the expression of an rpsD-lacZ fusion containing a UAG amber codon. This system permitted isolation of a conditional lethal mutant which required IPTG for growth.

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Year:  1994        PMID: 8144479      PMCID: PMC205319          DOI: 10.1128/jb.176.7.2108-2110.1994

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  14 in total

1.  Genetic location of the Bacillus subtilis sup-3 suppressor mutation.

Authors:  D J Henner; W Steinberg
Journal:  J Bacteriol       Date:  1979-08       Impact factor: 3.490

Review 2.  Transfer ribonucleic acid-mediated suppression of termination codons in Escherichia coli.

Authors:  G Eggertsson; D Söll
Journal:  Microbiol Rev       Date:  1988-09

3.  Construction of Escherichia coli amber suppressor tRNA genes. II. Synthesis of additional tRNA genes and improvement of suppressor efficiency.

Authors:  L G Kleina; J M Masson; J Normanly; J Abelson; J H Miller
Journal:  J Mol Biol       Date:  1990-06-20       Impact factor: 5.469

4.  Isolation of a suppressor mutant in Bacillus subtilis.

Authors:  S Okubo; T Yanagida
Journal:  J Bacteriol       Date:  1968-03       Impact factor: 3.490

5.  Catabolic repression of bacterial sporulation.

Authors:  P Schaeffer; J Millet; J P Aubert
Journal:  Proc Natl Acad Sci U S A       Date:  1965-09       Impact factor: 11.205

6.  Suppressor system in Bacillus subtilis 168.

Authors:  C P Georgopoulos
Journal:  J Bacteriol       Date:  1969-03       Impact factor: 3.490

7.  Amber suppression: a nucleotide change in the anticodon of a tyrosine transfer RNA.

Authors:  H M Goodman; J Abelson; A Landy; S Brenner; J D Smith
Journal:  Nature       Date:  1968-03-16       Impact factor: 49.962

8.  Bacillus subtilis mutant allele sup-3 causes lysine insertion at ochre codons: use of sup-3 in studies of translational attenuation.

Authors:  W W Mulbry; N P Ambulos; P S Lovett
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

9.  Two large clusters with thirty-seven transfer RNA genes adjacent to ribosomal RNA gene sets in Bacillus subtilis. Sequence and organization of trrnD and trrnE gene clusters.

Authors:  E F Wawrousek; N Narasimhan; J N Hansen
Journal:  J Biol Chem       Date:  1984-03-25       Impact factor: 5.157

10.  The Bacillus subtilis ochre suppressor sup-3 is located in an operon of seven tRNA genes.

Authors:  D B Garrity; S A Zahler
Journal:  J Bacteriol       Date:  1993-10       Impact factor: 3.490

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  4 in total

1.  tRNA determinants for transcription antitermination of the Bacillus subtilis tyrS gene.

Authors:  F J Grundy; J A Collins; S M Rollins; T M Henkin
Journal:  RNA       Date:  2000-08       Impact factor: 4.942

2.  Factors that influence T box riboswitch efficacy and tRNA affinity.

Authors:  C Zeng; S Zhou; S C Bergmeier; J V Hines
Journal:  Bioorg Med Chem       Date:  2015-07-16       Impact factor: 3.641

3.  Interaction between the acceptor end of tRNA and the T box stimulates antitermination in the Bacillus subtilis tyrS gene: a new role for the discriminator base.

Authors:  F J Grundy; S M Rollins; T M Henkin
Journal:  J Bacteriol       Date:  1994-08       Impact factor: 3.490

4.  High-throughput system for the presentation of secreted and surface-exposed proteins from Gram-positive bacteria in functional metagenomics studies.

Authors:  Dragana Dobrijevic; Gaetana Di Liberto; Kosei Tanaka; Tomas de Wouters; Rozenn Dervyn; Samira Boudebbouze; Johan Binesse; Hervé M Blottière; Alexandre Jamet; Emmanuelle Maguin; Maarten van de Guchte
Journal:  PLoS One       Date:  2013-06-14       Impact factor: 3.240

  4 in total

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