Literature DB >> 8144168

A type 1 DNA topoisomerase from the kinetoplast hemoflagellate Leishmania donovani.

A K Chakraborty1, A Gupta, H K Majumder.   

Abstract

A type 1 DNA topoisomerase has been purified from the nuclei of the kinetoplast hemoflagellate Leishmania donovani using polyethylene glycol fractionation and chromatography on hydroxylapatite, phosphocellulose and phenylsepharose column. The relaxation activity is ATP independent. Mg2+ is an essential cofactor for the reaction with an optimum at 10 mM. Mg2+ can be substituted by Mn2+ at 5 mM concentration. The relaxation reaction exhibits a salt optimum at 100 mM KCl. The enzyme can not remove supercoils from positive superhelical DNAs nor can induce supercoiling of relaxed DNAs. The topoisomerase activity is associated with a polypeptide of molecular weight about 67 kDa as shown by sephacryl-S200 gel filtration and by electrophoresis on sodium dodecyl sulphate-polyacrylamide gels.

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Year:  1993        PMID： 8144168

Source DB:  PubMed          Journal:  Indian J Biochem Biophys        ISSN： 0301-1208            Impact factor:   1.918


  5 in total

1.  An insight into the active site of a type I DNA topoisomerase from the kinetoplastid protozoan Leishmania donovani.

Authors:  Aditi Das; Chhabinath Mandal; Arindam Dasgupta; Tanushri Sengupta; Hemanta K Majumder
Journal:  Nucleic Acids Res       Date:  2002-02-01       Impact factor: 16.971

2.  Canine leishmaniosis: in vitro efficacy of miltefosine and marbofloxacin alone or in combination with allopurinol against clinical strains of Leishmania infantum.

Authors:  Anna Maria Farca; B Miniscalco; P Badino; R Odore; P Monticelli; A Trisciuoglio; E Ferroglio
Journal:  Parasitol Res       Date:  2012-01-05       Impact factor: 2.289

3.  An unusual type IB topoisomerase from African trypanosomes.

Authors:  Annette L Bodley; Asit K Chakraborty; Suji Xie; Christian Burri; Theresa A Shapiro
Journal:  Proc Natl Acad Sci U S A       Date:  2003-06-16       Impact factor: 11.205

4.  Molecular and cytotoxic effects of camptothecin, a topoisomerase I inhibitor, on trypanosomes and Leishmania.

Authors:  A L Bodley; T A Shapiro
Journal:  Proc Natl Acad Sci U S A       Date:  1995-04-25       Impact factor: 11.205

5.  Efficacy of a Binuclear Cyclopalladated Compound Therapy for Cutaneous Leishmaniasis in the Murine Model of Infection with Leishmania amazonensis and Its Inhibitory Effect on Topoisomerase 1B.

Authors:  Angela Maria Arenas Velásquez; Willian Campos Ribeiro; Vutey Venn; Silvia Castelli; Mariana Santoro de Camargo; Renata Pires de Assis; Rodrigo Alves de Souza; Aline Rimoldi Ribeiro; Thaís Gaban Passalacqua; João Aristeu da Rosa; Amanda Martins Baviera; Antonio Eduardo Mauro; Alessandro Desideri; Elmo Eduardo Almeida-Amaral; Marcia A S Graminha
Journal:  Antimicrob Agents Chemother       Date:  2017-07-25       Impact factor: 5.191
  5 in total

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