Literature DB >> 814378

Studies on the 12alpha and 26-hydroxylation of bile alcohols by rabbit liver microsomes.

G Nicolau, B I Cohen, G Salen, E H Mosbach.   

Abstract

12Alpha--Hydroxylation of two C27-steroids by rabbit liver microsomes was studied. Optimal assay conditions were determined with 7alpha-hydroxy-4-cholesten-3-one and 5beta-cholestand-3alpha, 7alpha-diol as substrates. The rate of 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one was found to be greater than that of 5beta-cholestane-3alpha, 7alpha-diol by ca. 60%. Microsomal 26-hydroxylation of 5beta-cholestane-3alpha7alpha-diol was also measured, and the ratio of 26-hydroxylation to 12alpha-hydroxylation of 5beta-cholestane-3alpha, 7alpha-diol was found to be ca. 0.4. Rabbit liver 12-alphahydroxylase was more active than that of three other species (man, rat, monkey), explaining in part the predominance of cholic acid in rabbit bile.

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Year:  1976        PMID： 814378     DOI： 10.1007/BF02532665

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  10 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

Review 2.  Hepatic synthesis of bile acids. Biochemical steps and mechanisms of rate control.

Authors:  E H Mosbach
Journal:  Arch Intern Med       Date:  1972-10

3.  On the properties of the 12 alpha-hydroxylase in cholic acid biosynthesis. Bile acids and steroids. 198.

Authors:  K Einarsson
Journal:  Eur J Biochem       Date:  1968-06

4.  On the effect of thyroid hormone on 26-hydroxylation of C 27 -steroids in rat liver.

Authors:  I Björkhem; H Danielsson; J Gustafsson
Journal:  FEBS Lett       Date:  1973-04-01       Impact factor: 4.124

5.  Omega-hydroxylation of steriod side-chain in biosynthesis of bile acids.

Authors:  I Björkhem; J Gustafsson
Journal:  Eur J Biochem       Date:  1973-07-02

Review 6.  Hydroxylations in biosynthesis and metabolism of bile acids.

Authors:  I Björkhem; H Danielsson
Journal:  Mol Cell Biochem       Date:  1974-09-30       Impact factor: 3.396

7.  On the synthesis and metabolism of cholest-4-en-7-alpha-ol-3-one. Bile acids and steroids 156.

Authors:  I Björkhem; H Danielsson; C Issidorides; A Kallner
Journal:  Acta Chem Scand       Date:  1965

8.  Hepatic cholesterol metabolism in patients with gallstones.

Authors:  G Salen; G Nicolau; S Shefer; E H Mosbach
Journal:  Gastroenterology       Date:  1975-09       Impact factor: 22.682

9.  SYNTHESIS AND METABOLISM OF CHOLEST-4-ENE-7-ALPHA,12-ALPHA-DIOL-3-ONE AND 5-BETA-CHOLESTANE-7-ALPHA,12-ALPHA-DIOL-3-ONE. BILE ACIDS AND STEROIDS 153.

Authors:  O BERSEUS; H DANIELSSON; A KALLNER
Journal:  J Biol Chem       Date:  1965-06       Impact factor: 5.157

10.  HMG CoA reductase of intestinal mucosa and liver of the rat.

Authors:  S Shefer; S Hauser; V Lapar; E H Mosbach
Journal:  J Lipid Res       Date:  1972-05       Impact factor: 5.922
  10 in total
  2 in total

1.  Biosynthesis of chenodeoxycholic acid in man: stereospecific side-chain hydroxylations of 5beta-cholestane-3alpha,7alpha-diol.

Authors:  S Shefer; F W Cheng; A K Batta; B Dayal; G S Tint; G Salen
Journal:  J Clin Invest       Date:  1978-09       Impact factor: 14.808

2.  Biosynthesis of bile acids in cerebrotendinous xanthomatosis. Relationship of bile acid pool sizes and synthesis rates to hydroxylations at C-12, C-25, and C-26.

Authors:  G Salen; S Shefer; G S Tint; G Nicolau; B Dayal; A K Batta
Journal:  J Clin Invest       Date:  1985-08       Impact factor: 14.808
  2 in total

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