Literature DB >> 8143311

How to isolate cardiac myocytes.

J Tytgat1.   

Abstract

A complete technique is described for the isolation of myocytes from mammalian hearts using the Langendorff perfusion technique. The use of calcium-free solution containing collagenase and protease, followed by low calcium solution, consistently results in a large number of calcium tolerant myocytes which are well suited for long periods of electrophysiological recording.

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Year:  1994        PMID： 8143311     DOI： 10.1093/cvr/28.2.280

Source DB:  PubMed          Journal:  Cardiovasc Res        ISSN： 0008-6363            Impact factor:   10.787


  14 in total

1.  Oxidative stress in the aging rat heart is reversed by dietary supplementation with (R)-(alpha)-lipoic acid.

Authors:  J H Suh; E T Shigeno; J D Morrow; B Cox; A E Rocha; B Frei; T M Hagen
Journal:  FASEB J       Date:  2001-03       Impact factor: 5.191

2.  Phosphorylation of the cAMP-dependent protein kinase (PKA) regulatory subunit modulates PKA-AKAP interaction, substrate phosphorylation, and calcium signaling in cardiac cells.

Authors:  Sabrina Manni; Joseph H Mauban; Christopher W Ward; Meredith Bond
Journal:  J Biol Chem       Date:  2008-06-12       Impact factor: 5.157

3.  Increasing DHA and EPA concentrations prolong action potential durations and reduce transient outward potassium currents in rat ventricular myocytes.

Authors:  Hong-Xia Li; Ru-Xing Wang; Xiao-Rong Li; Tao Guo; Ying Wu; Su-Xia Guo; Li-Ping Sun; Zhen-Yu Yang; Xiang-Jun Yang; Wen-Ping Jiang
Journal:  Lipids       Date:  2010-12-08       Impact factor: 1.880

4.  Blocking effect of salvianolic acid A on calcium channels in isolated rat ventricular myocytes.

Authors:  Bao Wang; Jian-xun Liu; Hong-xu Meng; Cheng-ren Lin
Journal:  Chin J Integr Med       Date:  2011-04-26       Impact factor: 1.978

5.  Effects of Xinjining extract on inward rectifier potassium current in ventricular myocytes of guinea pig.

Authors:  Ming-jun Zhu; Guo-juan Wang; Yong-xia Wang; Jie-lin Pu; Hong-jun Liu; Hai-bin Yu
Journal:  Chin J Integr Med       Date:  2010-02-04       Impact factor: 1.978

6.  Docosahexaenoic acid has influence on action potentials and transient outward potassium currents of ventricular myocytes.

Authors:  Ru-xing Wang; Xiao-rong Li; Tao Guo; Li-ping Sun; Su-xia Guo; Zhen-Yu Yang; Xiang-Jun Yang; Wen-ping Jiang
Journal:  Lipids Health Dis       Date:  2010-04-17       Impact factor: 3.876

7.  Swimming training can affect intrinsic calcium current characteristics in rat myocardium.

Authors:  Sen Wang; Ji Zheng Ma; Shu Shu Zhu; Dong Jie Xu; Jian Gang Zou; Ke Jiang Cao
Journal:  Eur J Appl Physiol       Date:  2008-07-02       Impact factor: 3.078

8.  Effects of wenxin keli on the action potential and L-type calcium current in rats with transverse aortic constriction-induced heart failure.

Authors:  Yu Chen; Yang Li; Lili Guo; Wen Chen; Mingjing Zhao; Yonghong Gao; Aiming Wu; Lixia Lou; Jie Wang; Xiaoqiu Liu; Yanwei Xing
Journal:  Evid Based Complement Alternat Med       Date:  2013-11-11       Impact factor: 2.629

9.  Effects of allitridum on the transient outward potassium current in rats with heart failure.

Authors:  Xiao-Jing Zhao; Kun Lin; Yu Zhang; Bin Xu; Li Liu; Yi-Cheng Fu; Xi Chen; Zhong-Qi Cai; Zhi-Juan Wu; Yun Huang; Yang Li
Journal:  J Geriatr Cardiol       Date:  2016-09       Impact factor: 3.327

10.  21st Century Cardio-Oncology: Identifying Cardiac Safety Signals in the Era of Personalized Medicine.

Authors:  Calvin Chen Sheng; Laleh Amiri-Kordestani; Todd Palmby; Thomas Force; Charles C Hong; Joseph C Wu; Kevin Croce; Geoffrey Kim; Javid Moslehi
Journal:  JACC Basic Transl Sci       Date:  2016-08
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