D Sakkas1, N Jaquenoud, G Leppens, A Campana. 1. Clinique de Stérilité et d'Endocrinologie Gynécologique, Hôpital Cantonal Universitaire de Genève, Switzerland.
Abstract
OBJECTIVE: To investigate whether coculture of human embryos on Vero cells improves embryo viability compared with a routine culture method. SETTING: In vitro fertilization Clinic of the Hôpital Cantonal Universitaire de Genève, Geneva, Switzerland. PATIENT SELECTION: Couples who had given informed consent, had undergone < 3 IVF cycles with ET and where the male had normal semen parameters were selected. Patients who had undergone > or = 3 IVF cycles with ET were allocated directly to coculture. DESIGN: Patients were randomly allocated to have their embryos cultured in a routine embryo culture medium or in coculture with Vero cells. RESULTS: There was no difference in pregnancy rates between the two culture groups. Coculture gave a high (> 50%) rate of blastocyst formation. In 16 cycles where patients had previously undergone > or = 3 IVF cycles 4 patients became pregnant. CONCLUSIONS: Coculture provides no benefit for patients that are performing their initial treatment cycles in IVF.
RCT Entities:
OBJECTIVE: To investigate whether coculture of human embryos on Vero cells improves embryo viability compared with a routine culture method. SETTING: In vitro fertilization Clinic of the Hôpital Cantonal Universitaire de Genève, Geneva, Switzerland. PATIENT SELECTION: Couples who had given informed consent, had undergone < 3 IVF cycles with ET and where the male had normal semen parameters were selected. Patients who had undergone > or = 3 IVF cycles with ET were allocated directly to coculture. DESIGN:Patients were randomly allocated to have their embryos cultured in a routine embryo culture medium or in coculture with Vero cells. RESULTS: There was no difference in pregnancy rates between the two culture groups. Coculture gave a high (> 50%) rate of blastocyst formation. In 16 cycles where patients had previously undergone > or = 3 IVF cycles 4 patients became pregnant. CONCLUSIONS: Coculture provides no benefit for patients that are performing their initial treatment cycles in IVF.
Authors: M J Tucker; H I Kort; A A Toledo; P C Morton; G Wright; P E Ingargiola; C L Sweitzer Journal: J Assist Reprod Genet Date: 1995-11 Impact factor: 3.412
Authors: L I Barmat; H C Liu; S D Spandorfer; A Kowalik; C Mele; K Xu; L Veeck; M Damario; Z Rosenwaks Journal: J Assist Reprod Genet Date: 1999-03 Impact factor: 3.412