OBJECTIVE: To determine the effect of insulin-like growth factor-II (IGF-II) on human granulosa cell proliferation in vitro. DESIGN: Thymidine incorporation and cell count were determined to assess human granulosa cell proliferation. SETTING: The study was performed in an academic research environment. PATIENTS: Granulosa cells were obtained from women undergoing IVF procedures. INTERVENTIONS: Granulosa cells were dispersed enzymatically and cultured in both serum-containing and serum-free medium. MAIN OUTCOME MEASURE: Granulosa cell proliferation in basal conditions and after stimulation with IGF-II given alone or in combination with basic fibroblast growth factor (basic FGF). RESULTS: Treatment of human granulosa cells with IGF-II for 24 hours significantly increased thymidine incorporation. Furthermore, this mitogenic effect was confirmed when granulosa cells were cultured in presence of IGF-II for 7 to 10 days and their proliferation was evaluated by cell counting. The association of IGF-II and basic FGF did not result in a synergistic or additive effect. CONCLUSION: As already demonstrated in other species, IGF-II also is able to stimulate human granulosa cell proliferation in vitro. Thus, it might be considered among the intraovarian modulators of granulosa cell physiology.
OBJECTIVE: To determine the effect of insulin-like growth factor-II (IGF-II) on human granulosa cell proliferation in vitro. DESIGN:Thymidine incorporation and cell count were determined to assess human granulosa cell proliferation. SETTING: The study was performed in an academic research environment. PATIENTS: Granulosa cells were obtained from women undergoing IVF procedures. INTERVENTIONS: Granulosa cells were dispersed enzymatically and cultured in both serum-containing and serum-free medium. MAIN OUTCOME MEASURE: Granulosa cell proliferation in basal conditions and after stimulation with IGF-II given alone or in combination with basic fibroblast growth factor (basic FGF). RESULTS: Treatment of human granulosa cells with IGF-II for 24 hours significantly increased thymidine incorporation. Furthermore, this mitogenic effect was confirmed when granulosa cells were cultured in presence of IGF-II for 7 to 10 days and their proliferation was evaluated by cell counting. The association of IGF-II and basic FGF did not result in a synergistic or additive effect. CONCLUSION: As already demonstrated in other species, IGF-II also is able to stimulate human granulosa cell proliferation in vitro. Thus, it might be considered among the intraovarian modulators of granulosa cell physiology.
Authors: Sarah C Baumgarten; Scott M Convissar; A Musa Zamah; Michelle A Fierro; Nicola J Winston; Bert Scoccia; Carlos Stocco Journal: J Clin Endocrinol Metab Date: 2015-06-12 Impact factor: 5.958
Authors: Rebecca S Brogan; Scott Mix; Muraly Puttabyatappa; Catherine A VandeVoort; Charles L Chaffin Journal: Fertil Steril Date: 2009-02-24 Impact factor: 7.329